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Literature summary for 1.14.13.B28 extracted from

  • Oku, Y.; Ohtaki, A.; Kamitori, S.; Nakamura, N.; Yohda, M.; Ohno, H.; Kawarabayasi, Y.
    Structure and direct electrochemistry of cytochrome P450 from the thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain 7 (2004), J. Inorg. Biochem., 98, 1194-1199 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
vapor diffusion method using a protein solution (15 mg/ml) and a reservoir solution (30% (w/v) polyethylene glycol 4000, 200 mM lithium sulfate monohydrate and 100 mM Tris-HCl, pH 8.5) Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q972I2
-
-
Sulfurisphaera tokodaii 7 Q972I2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information electron-transfer reaction between the electrode and heme iron. Direct electrochemistry of P450st in a didodecyldimethylammonium bromide film on a plastic formed carbon electrode is demonstrated. A quasi-reversible redox response is observed at temperatures of up to 80°C Sulfurisphaera tokodaii ?
-
?
additional information electron-transfer reaction between the electrode and heme iron. Direct electrochemistry of P450st in a didodecyldimethylammonium bromide film on a plastic formed carbon electrode is demonstrated. A quasi-reversible redox response is observed at temperatures of up to 80°C Sulfurisphaera tokodaii 7 ?
-
?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
CYP119
-
Sulfurisphaera tokodaii
cytochrome P450 119
-
Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80 a clear redox response is observed at even 80°C Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
stable at Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
heme electron-transfer reaction between the electrode and heme iron Sulfurisphaera tokodaii