Literature summary for 1.14.13.B28 extracted from

McLean, M.A.; Maves, S.A.; Weiss, K.A.; Krepich, S.; Sligar, S.G.
Characterization of a cytochrome P450 from the acidothermophilic archaea Sulfolobus solfataricus (1998), Biochem. Biophys. Res. Commun., 252, 166-172 .

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Crystallization (Commentary)

Crystallization (Comment)	Organism
molecular modeling of structure	Saccharolobus solfataricus

General Stability

General Stability	Organism
exhibits remarkable thermo- and pressure stability, i.e. a hydrostatic pressure up to 2 kbar	Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
using a heat denaturation step, ammonium sulfate precipitation (30-50%), a size exclusion column, and an HPLC anion exchange column	Saccharolobus solfataricus

Synonyms

Synonyms	Comment	Organism
CYP119	-	Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
91	-	melting temperature, irreversible	Saccharolobus solfataricus

Cofactor

Cofactor	Comment	Organism	Structure
heme	substrate free oxidized form of the protein shows a Soret maximum at 415 nm and visible bands at 533 and 566 nm. In the reduced form the Soret band blue shifts to 410 nm	Saccharolobus solfataricus

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Release 2023.1 (January 2023)