Cloned (Comment) | Organism |
---|---|
gene pikC, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptomyces venezuelae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of an enzyme deletion mutant strain HK954, showing blocked biosynthesis of endogenous macrolactone aglycones | Streptomyces venezuelae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
macrolide YC-17 + [reduced NADPH-hemoprotein reductase] + O2 | Streptomyces venezuelae | Hydroxylation of C-10 position yield methymycin and of the C-12 neomethymycin. Hydroxylation at both positions yields novamethymycin. | methymycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
macrolide YC-17 + [reduced NADPH-hemoprotein reductase] + O2 | Streptomyces venezuelae | Hydroxylation of C-10 position yield methymycin and of the C-12 neomethymycin. Hydroxylation at both positions yields novamethymycin. | neomethymycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
macrolide YC-17 + [reduced NADPH-hemoprotein reductase] + O2 | Streptomyces venezuelae | Hydroxylation of C-10 position yield methymycin and of the C-12 neomethymycin. Hydroxylation at both positions yields novamethymycin. | novamethymycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | Streptomyces venezuelae | the enzyme is involved in macrolide biosynthesis and performs unusual C4-hydroxylation | ? | - |
? | |
narbomycin + [reduced NADPH-hemoprotein reductase] + O2 | Streptomyces venezuelae | the organism is a pikromycin producer | pikromycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces venezuelae | - |
strain ATCC 15439 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged PikC from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity | Streptomyces venezuelae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
macrolide YC-17 + [reduced NADPH-hemoprotein reductase] + O2 | Hydroxylation of C-10 position yield methymycin and of the C-12 neomethymycin. Hydroxylation at both positions yields novamethymycin. | Streptomyces venezuelae | methymycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
macrolide YC-17 + [reduced NADPH-hemoprotein reductase] + O2 | Hydroxylation of C-10 position yield methymycin and of the C-12 neomethymycin. Hydroxylation at both positions yields novamethymycin. | Streptomyces venezuelae | neomethymycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
macrolide YC-17 + [reduced NADPH-hemoprotein reductase] + O2 | Hydroxylation of C-10 position yield methymycin and of the C-12 neomethymycin. Hydroxylation at both positions yields novamethymycin. | Streptomyces venezuelae | novamethymycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
additional information | the enzyme is involved in macrolide biosynthesis and performs unusual C4-hydroxylation | Streptomyces venezuelae | ? | - |
? | |
additional information | structural diversification of macrolactones by the substrate-flexible enzyme, substrate specificity, degree of plasticity towards alternative substrates, overview, modeling of macrolide conformations and enzyme-substrate complex | Streptomyces venezuelae | ? | - |
? | |
narbomycin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces venezuelae | pikromycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
narbomycin + [reduced NADPH-hemoprotein reductase] + O2 | the organism is a pikromycin producer | Streptomyces venezuelae | pikromycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
oleandomycin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Streptomyces venezuelae | 4-hydroxy-oleandomycin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cytochrome P450 monooxygenase | - |
Streptomyces venezuelae |
PikC | - |
Streptomyces venezuelae |
PikC hydroxylase | - |
Streptomyces venezuelae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces venezuelae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Streptomyces venezuelae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Streptomyces venezuelae |