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Literature summary for 1.14.14.1 extracted from

  • Siminszky, B.; Gavilano, L.; Bowen, S.W.; Dewey, R.E.
    Conversion of nicotine to nornicotine in Nicotiana tabacum is mediated by CYP82E4, a cytochrome P450 monooxygenase (2005), Proc. Natl. Acad. Sci. USA, 102, 14919-14924.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.1

Cloned(Commentary)

Cloned (Comment) Organism
CYP82E2, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants Nicotiana tabacum
CYP82E3, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants Nicotiana tabacum
CYP82E4v1, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11, overexpression in transgenic tobacco plants Nicotiana tabacum
CYP82E4v2, DNA and amino acid sequence determination and analysis, microarray-based strategy to identify genes that are differentially regulated between closely related tobacco lines that accumulate either nicotine, termed nonconverters, or nornicotine, termed converters, as the predominant alkaloid in the cured leaf, expression in Saccharomyces cerevisiae strain WAT11 Nicotiana tabacum

Protein Variants

Protein Variants Comment Organism
additional information construction of transgenic plants overexpressing the enzyme, analysis of alkaloid spectrum, overview Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
 Nicotiana tabacum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Nicotiana tabacum the enzyme demethylates nicotine to nornicotine, nornicotine serves as the precursor in the synthesis of the well characterized carcinogen N'-nitrosonornicotine during the curing and processing of tobacco ?
-
 ?

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q38Q84 CYP82E3
-
Nicotiana tabacum Q38Q85 CYP82E2
-
Nicotiana tabacum Q38Q86 CYP82E4v1
-
Nicotiana tabacum Q38Q87 CYP82E4v2; three CYP82E isozymes
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme demethylates nicotine to nornicotine, nornicotine serves as the precursor in the synthesis of the well characterized carcinogen N'-nitrosonornicotine during the curing and processing of tobacco Nicotiana tabacum ?
-
 ?
additional information the enzyme demethylates nicotine to nornicotine Nicotiana tabacum ?
-
 ?

Synonyms

Synonyms Comment Organism
CYP82E2
-
 Nicotiana tabacum
CYP82E3
-
 Nicotiana tabacum
CYP82E4v1
-
 Nicotiana tabacum
CYP82E4v2
-
 Nicotiana tabacum
cytochrome P450 monooxygenase
-
 Nicotiana tabacum
More the enzyme belongs to the CYP82E2 family Nicotiana tabacum