Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli NovaBlue(DE3) | Rhodococcus ruber |
Protein Variants | Comment | Organism |
---|---|---|
A86T/T91S/A109F/I179F/I267L | site-specific mutagenesis, mutant 70A08 shows 150fold increased dealkylation activity compared to the wild-type enzyme | Rhodococcus ruber |
additional information | site-specific mutagenesis and screening for a gain of function mutant of CYP116B3, dealkylation activity of 12 variants chosen from the primary screening, overview. Identification of a mutant 74H10 that shows a 240fold increased deethylation activity toward 7-ethoxycoumarin and a 10fold increased demethylation activity toward 7-methoxycoumarin | Rhodococcus ruber |
T91S/A109L/I179F/I267L | site-specific mutagenesis, mutant 74H10 shows 240fold increased dealkylation activity compared to the wild-type enzyme | Rhodococcus ruber |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme protein | Rhodococcus ruber |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-ethoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2 | Rhodococcus ruber | - |
7-hydroxycoumarin + [oxidized NADPH-hemoprotein reductase] + H2O + ? | - |
? | |
7-methoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2 | Rhodococcus ruber | - |
7-hydroxycoumarin + [oxidized NADPH-hemoprotein reductase] + ? | - |
? | |
additional information | Rhodococcus ruber | YP116B3 catalyzes the dealkylation of 7-ethoxycoumarin and the hydroxylation of substituted and unsubstituted aromatics, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus ruber | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-ethoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus ruber | 7-hydroxycoumarin + [oxidized NADPH-hemoprotein reductase] + H2O + ? | - |
? | |
7-methoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus ruber | 7-hydroxycoumarin + [oxidized NADPH-hemoprotein reductase] + ? | - |
? | |
additional information | YP116B3 catalyzes the dealkylation of 7-ethoxycoumarin and the hydroxylation of substituted and unsubstituted aromatics, overview | Rhodococcus ruber | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP116B3 | - |
Rhodococcus ruber |
cytochrome P450 monooxygenase 116B3 | - |
Rhodococcus ruber |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rhodococcus ruber |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rhodococcus ruber |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | structure modeling of the heme domain of CYP116B3 | Rhodococcus ruber | |
NADH | - |
Rhodococcus ruber |
General Information | Comment | Organism |
---|---|---|
additional information | multifunctional properties of versatile P450s from the basidiomycete | Rhodococcus ruber |