Any feedback?
Literature summary for 1.14.14.1 extracted from
- Engineering cytochrome P450 monooxygenase CYP 116B3 for high dealkylation activity (2010), Biotechnol. Lett., 32, 841-845.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant enzymes in Escherichia coli NovaBlue(DE3) | Rhodococcus ruber |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A86T/T91S/A109F/I179F/I267L | site-specific mutagenesis, mutant 70A08 shows 150fold increased dealkylation activity compared to the wild-type enzyme | Rhodococcus ruber |
| additional information | site-specific mutagenesis and screening for a gain of function mutant of CYP116B3, dealkylation activity of 12 variants chosen from the primary screening, overview. Identification of a mutant 74H10 that shows a 240fold increased deethylation activity toward 7-ethoxycoumarin and a 10fold increased demethylation activity toward 7-methoxycoumarin | Rhodococcus ruber |
| T91S/A109L/I179F/I267L | site-specific mutagenesis, mutant 74H10 shows 240fold increased dealkylation activity compared to the wild-type enzyme | Rhodococcus ruber |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | heme protein | Rhodococcus ruber |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-ethoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2 | Rhodococcus ruber | - |
7-hydroxycoumarin + [oxidized NADPH-hemoprotein reductase] + H2O + ? | - |
? | |
| 7-methoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2 | Rhodococcus ruber | - |
7-hydroxycoumarin + [oxidized NADPH-hemoprotein reductase] + ? | - |
? | |
| additional information | Rhodococcus ruber | YP116B3 catalyzes the dealkylation of 7-ethoxycoumarin and the hydroxylation of substituted and unsubstituted aromatics, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodococcus ruber | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7-ethoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus ruber | 7-hydroxycoumarin + [oxidized NADPH-hemoprotein reductase] + H2O + ? | - |
? | |
| 7-methoxycoumarin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Rhodococcus ruber | 7-hydroxycoumarin + [oxidized NADPH-hemoprotein reductase] + ? | - |
? | |
| additional information | YP116B3 catalyzes the dealkylation of 7-ethoxycoumarin and the hydroxylation of substituted and unsubstituted aromatics, overview | Rhodococcus ruber | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CYP116B3 | - |
Rhodococcus ruber |
| cytochrome P450 monooxygenase 116B3 | - |
Rhodococcus ruber |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Rhodococcus ruber |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Rhodococcus ruber |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | structure modeling of the heme domain of CYP116B3 | Rhodococcus ruber | |
| NADH | - |
Rhodococcus ruber | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | multifunctional properties of versatile P450s from the basidiomycete | Rhodococcus ruber |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
