BRENDA - Enzyme Database show
show all sequences of 1.14.14.101

Enzymic 12-hydroxylation and 12-O-methylation of dihydrochelirubine in dihydromacarpine formation by Thalictrum bulgaricum

Kammerer, L.; De-Eknamkul, W.; Zenk, M.H.; Phytochemistry 36, 1409-1416 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-Methyl-1,2-di(3-pyridyl)-1-propanone
i.e. methyrapone
Thalictrum bulgaricum
5-hydroxy-1,4-naphthoquinone
i.e. juglone
Thalictrum bulgaricum
CO
inhibition in the dark, effect is partly reversible upon illumination
Thalictrum bulgaricum
cytochrome c
-
Thalictrum bulgaricum
ketoconazole
-
Thalictrum bulgaricum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
associated with
Thalictrum bulgaricum
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
Thalictrum bulgaricum
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thalictrum bulgaricum
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
yeast-elicited cells
Thalictrum bulgaricum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
-
7393
Thalictrum bulgaricum
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7393
Thalictrum bulgaricum
-
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
7393
Thalictrum bulgaricum
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Thalictrum bulgaricum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Thalictrum bulgaricum
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
dependent on
Thalictrum bulgaricum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
dependent on
Thalictrum bulgaricum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-Methyl-1,2-di(3-pyridyl)-1-propanone
i.e. methyrapone
Thalictrum bulgaricum
5-hydroxy-1,4-naphthoquinone
i.e. juglone
Thalictrum bulgaricum
CO
inhibition in the dark, effect is partly reversible upon illumination
Thalictrum bulgaricum
cytochrome c
-
Thalictrum bulgaricum
ketoconazole
-
Thalictrum bulgaricum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
associated with
Thalictrum bulgaricum
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
Thalictrum bulgaricum
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
yeast-elicited cells
Thalictrum bulgaricum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
-
7393
Thalictrum bulgaricum
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
7393
Thalictrum bulgaricum
-
dihydrochelirubine + [reduced NADPH-hemoprotein reductase] + O2
involved the penultimate step in the biosynthesis of macarpine, the most highly oxidized benzophenanthridine alkaloid found in nature
7393
Thalictrum bulgaricum
12-hydroxydihydrochelirubine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
-
Thalictrum bulgaricum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
-
Thalictrum bulgaricum
Other publictions for EC 1.14.14.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
7393
Kammerer
-
Enzymic 12-hydroxylation and 1 ...
Thalictrum bulgaricum
Phytochemistry
36
1409-1416
1994
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5
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1
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1
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1
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1
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2
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1
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1
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1
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1
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5
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1
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1
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1
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2
-
1
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1
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