BRENDA - Enzyme Database show
show all sequences of 1.14.14.102

Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis

Pauli, H.H.; Kutchan, T.M.; Plant J. 13, 793-801 (1998)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Saccharomyces cerevisiae and Spodoptera frugiperda
Eschscholzia californica
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
(S)-N-methylcoclaurine
-
Eschscholzia californica
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
Eschscholzia californica
-
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Eschscholzia californica
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Eschscholzia californica
-
california poppy
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
285356
Eschscholzia californica
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285356
Eschscholzia californica
?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
absolute requirement for an N-methyl group, a 4'-hydroxyl moiety and S-configuration at carbon atom 1
285356
Eschscholzia californica
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285356
Eschscholzia californica
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Eschscholzia californica
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
Tris-HCl buffer
Eschscholzia californica
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Saccharomyces cerevisiae and Spodoptera frugiperda
Eschscholzia californica
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.015
-
(S)-N-methylcoclaurine
-
Eschscholzia californica
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
Eschscholzia californica
-
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Eschscholzia californica
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
285356
Eschscholzia californica
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285356
Eschscholzia californica
?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
absolute requirement for an N-methyl group, a 4'-hydroxyl moiety and S-configuration at carbon atom 1
285356
Eschscholzia californica
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
285356
Eschscholzia californica
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Eschscholzia californica
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
Tris-HCl buffer
Eschscholzia californica
Other publictions for EC 1.14.14.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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701436
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Molecular cloning and sequence ...
Coptis chinensis
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39
419-423
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1
1
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1
1
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1
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1
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1
1
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688821
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Metabolic engineering with a m ...
Papaver somniferum
Metab. Eng.
9
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2007
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1
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3
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1
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670561
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Papaver somniferum
Plant J.
47
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2006
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1
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2
1
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1
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3
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1
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3
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1
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1
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3
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660256
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Papaver somniferum
Plant Physiol.
138
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2005
1
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1
1
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2
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1
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1
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1
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660086
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Distribution of morphinan and ...
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Phytochemistry
53
555-564
2000
-
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-
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4
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8
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8
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4
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4
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8
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4
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-
285356
Pauli
Molecular cloning and function ...
Eschscholzia californica
Plant J.
13
793-801
1998
-
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1
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1
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1
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5
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2
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1
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1
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