BRENDA - Enzyme Database show
show all sequences of 1.14.14.102

The role of phloem sieve elements and laticifers in the biosynthesis and accumulation of alkaloids in opium poppy

Samanani, N.; Alcantara, J.; Bourgault, R.; Zulak, K.G.; Facchini, P.J.; Plant J. 47, 547-563 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
heterologous expression
Papaver somniferum
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum membrane
membrane-bound, in sieve element ER
Papaver somniferum
5789
-
additional information
subcellular localization study
Papaver somniferum
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
P450-dependent hydroxylase
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
the catalyzes step 5 in the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy, biosynthetic pathway leading from L-DOPA to morphine, overview
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
-
cv. Louisiana
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Papaver somniferum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
phloem
localization in the sieve elements adjacent or proximal to laticifers in the root and hypocotyl of opium poppy seedlings
Papaver somniferum
-
root
phloem
Papaver somniferum
-
seedling
root phloem, the abundance of the enzyme increases rapidly between 1 and 3 days after seed germination
Papaver somniferum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
670561
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
the catalyzes step 5 in the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy, biosynthetic pathway leading from L-DOPA to morphine, overview
670561
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
P450-dependent hydroxylase
Papaver somniferum
Cloned(Commentary) (protein specific)
Commentary
Organism
heterologous expression
Papaver somniferum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
P450-dependent hydroxylase
Papaver somniferum
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum membrane
membrane-bound, in sieve element ER
Papaver somniferum
5789
-
additional information
subcellular localization study
Papaver somniferum
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
P450-dependent hydroxylase
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
the catalyzes step 5 in the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy, biosynthetic pathway leading from L-DOPA to morphine, overview
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Papaver somniferum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
phloem
localization in the sieve elements adjacent or proximal to laticifers in the root and hypocotyl of opium poppy seedlings
Papaver somniferum
-
root
phloem
Papaver somniferum
-
seedling
root phloem, the abundance of the enzyme increases rapidly between 1 and 3 days after seed germination
Papaver somniferum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
670561
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
the catalyzes step 5 in the unique, cell type-specific biosynthesis of benzylisoquinoline alkaloids in the opium poppy, biosynthetic pathway leading from L-DOPA to morphine, overview
670561
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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670561
Samanani
The role of phloem sieve eleme ...
Papaver somniferum
Plant J.
47
547-563
2006
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1
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2
1
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1
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3
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1
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3
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2
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1
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1
1
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1
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3
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660256
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Papaver somniferum
Plant Physiol.
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2005
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Molecular cloning and function ...
Eschscholzia californica
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1
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5
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2
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1
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1
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