BRENDA - Enzyme Database show
show all sequences of 1.14.14.102

Metabolic engineering with a morphine biosynthetic P450 in opium poppy surpasses breeding

Frick, S.; Kramell, R.; Kutchan, T.M.; Metab. Eng. 9, 169-176 (2007)

Data extracted from this reference:

Application
Application
Commentary
Organism
biotechnology
the enzyme provides practical means to genetically engineer valuable secondary metabolites in this important medicinal plant
Papaver somniferum
Cloned(Commentary)
Commentary
Organism
gene cyp80b3, overexpression in Papaver somniferum plants, expression of anti-sense constructs, construction of transgenic plants using the Agrobacterium tumefaciens, strain AGL1, transfection method, expression of antisense cyp80b3 leads to a reduction of total alkaloid in latex up to 84%, phenotype, overview, enzyme overexpression results in an up to 450% increase in the amount of total alkaloid in latex without changing the ratio of the individual alkaloids, or together with an overall increase in the ratio of morphine
Papaver somniferum
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
a cytochrome P450 enzyme
Papaver somniferum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
the enzyme catalyzes a key regulation step in morphine biosynthesis
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Papaver somniferum
-
cv. C048-6-14-64
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
688821
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a key regulation step in morphine biosynthesis
688821
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
dependent on
Papaver somniferum
Application (protein specific)
Application
Commentary
Organism
biotechnology
the enzyme provides practical means to genetically engineer valuable secondary metabolites in this important medicinal plant
Papaver somniferum
Cloned(Commentary) (protein specific)
Commentary
Organism
gene cyp80b3, overexpression in Papaver somniferum plants, expression of anti-sense constructs, construction of transgenic plants using the Agrobacterium tumefaciens, strain AGL1, transfection method, expression of antisense cyp80b3 leads to a reduction of total alkaloid in latex up to 84%, phenotype, overview, enzyme overexpression results in an up to 450% increase in the amount of total alkaloid in latex without changing the ratio of the individual alkaloids, or together with an overall increase in the ratio of morphine
Papaver somniferum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
dependent on
Papaver somniferum
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
a cytochrome P450 enzyme
Papaver somniferum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
Papaver somniferum
the enzyme catalyzes a key regulation step in morphine biosynthesis
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
-
688821
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(S)-N-methylcoclaurine + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a key regulation step in morphine biosynthesis
688821
Papaver somniferum
(S)-3'-hydroxy-N-methylcoclaurine + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.102
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745008
Yang
Digital gene expression analy ...
Nelumbo nucifera
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8
80
2017
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720710
Desgagne-Penix
Systematic silencing of benzyl ...
Papaver somniferum
Plant J.
72
331-344
2012
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687887
Cho
Synergistic effects of sequent ...
Eschscholzia californica
J. Biotechnol.
135
117-122
2008
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701436
Zhou
-
Molecular cloning and sequence ...
Coptis chinensis
Zhong Cao Yao
39
419-423
2008
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1
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688821
Frick
Metabolic engineering with a m ...
Papaver somniferum
Metab. Eng.
9
169-176
2007
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670561
Samanani
The role of phloem sieve eleme ...
Papaver somniferum
Plant J.
47
547-563
2006
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660256
Alcantara
Sanguinarine biosynthesis is a ...
Papaver somniferum
Plant Physiol.
138
173-183
2005
1
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660086
Huang
Distribution of morphinan and ...
Papaver bracteatum, Papaver orientale, Papaver rhoeas, Papaver somniferum
Phytochemistry
53
555-564
2000
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285356
Pauli
Molecular cloning and function ...
Eschscholzia californica
Plant J.
13
793-801
1998
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1
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5
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