BRENDA - Enzyme Database show
show all sequences of 1.14.14.103

Light-induced cytochrome P450-dependent enzyme in indole alkaloid biosynthesis: tabersonine 16-hydroxylase

Schroder, G.; Unterbusch, E.; Kaltenbach, M.; Schmidt, J.; Strack, D.; De Luca, V.; Schroder, J.; FEBS Lett. 458, 97-102 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Catharanthus roseus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
129000
-
SDS-PAGE
Catharanthus roseus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tabersonine + NADPH + H+ + O2
Catharanthus roseus
indole alkaloid synthesis
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
P98183
line CP3a
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tabersonine + NADPH + H+ + O2
-
438775
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
tabersonine + NADPH + H+ + O2
indole alkaloid synthesis
438775
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
K
Catharanthus roseus
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
K
Catharanthus roseus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
129000
-
SDS-PAGE
Catharanthus roseus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tabersonine + NADPH + H+ + O2
Catharanthus roseus
indole alkaloid synthesis
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tabersonine + NADPH + H+ + O2
-
438775
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
tabersonine + NADPH + H+ + O2
indole alkaloid synthesis
438775
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Other publictions for EC 1.14.14.103
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743890
Liu
Enhancement of vindoline and ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2014
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
728525
Besseau
A pair of tabersonine 16-hydro ...
Catharanthus roseus
Plant Physiol.
163
1792-1803
2013
-
-
1
-
-
-
-
2
1
-
-
4
-
2
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
1
-
-
4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712866
Guirimand
Spatial organization of the vi ...
Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
-
-
1
-
-
-
-
-
3
-
-
1
-
3
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
676372
Magnotta
Identification of a low vindol ...
Catharanthus roseus
Phytochemistry
67
1758-1764
2006
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
-
-
3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
670558
Murata
localization of tabersonine 16 ...
Catharanthus roseus
Plant J.
44
581-594
2005
-
-
1
-
-
-
-
-
-
-
-
2
-
3
-
-
-
-
-
4
-
-
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
4
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
438775
Schroder
Light-induced cytochrome P450- ...
Catharanthus roseus
FEBS Lett.
458
97-102
1999
-
-
1
-
-
-
-
-
-
-
1
1
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438776
St-Pierre
A cytochrome P-450 monooxygena ...
Catharanthus roseus
Plant Physiol.
109
131-139
1995
-
-
-
-
-
-
6
2
1
-
-
1
-
3
-
-
-
-
-
4
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
2
1
-
-
1
-
-
-
-
-
4
-
-
2
-
1
-
-
-
2
-
-
-
-
-
-
-
-
-