BRENDA - Enzyme Database show
show all sequences of 1.14.14.103

Identification of a low vindoline accumulating cultivar of Catharanthus roseus (L.) G. Don by alkaloid and enzymatic profiling

Magnotta, M.; Murata, J.; Chen, J.; De Luca, V.; Phytochemistry 67, 1758-1764 (2006)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Catharanthus roseus
alkaloid and enzymatic profiling of the vindoline biosynthetic pathway, overview
?
-
-
-
tabersonine + NADPH + H+ + O2
Catharanthus roseus
step in monoterpenoid indole alkaloid biosynthesis, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
-
50 different flowering cultivars, one low vindoline accumulating cultivar Little Delicata
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
young
Catharanthus roseus
-
seedling
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
alkaloid and enzymatic profiling of the vindoline biosynthetic pathway, overview
676372
Catharanthus roseus
?
-
-
-
-
tabersonine + NADPH + H+ + O2
-
676372
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
tabersonine + NADPH + H+ + O2
step in monoterpenoid indole alkaloid biosynthesis, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
676372
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Catharanthus roseus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Catharanthus roseus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Catharanthus roseus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Catharanthus roseus
alkaloid and enzymatic profiling of the vindoline biosynthetic pathway, overview
?
-
-
-
tabersonine + NADPH + H+ + O2
Catharanthus roseus
step in monoterpenoid indole alkaloid biosynthesis, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
young
Catharanthus roseus
-
seedling
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
alkaloid and enzymatic profiling of the vindoline biosynthetic pathway, overview
676372
Catharanthus roseus
?
-
-
-
-
tabersonine + NADPH + H+ + O2
-
676372
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
tabersonine + NADPH + H+ + O2
step in monoterpenoid indole alkaloid biosynthesis, biosynthesis of anticancer dimeric alkaloids vinblastine and vincristine, overview
676372
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Catharanthus roseus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Catharanthus roseus
Other publictions for EC 1.14.14.103
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743890
Liu
Enhancement of vindoline and ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2014
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1
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2
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2
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1
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2
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1
1
1
1
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728525
Besseau
A pair of tabersonine 16-hydro ...
Catharanthus roseus
Plant Physiol.
163
1792-1803
2013
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1
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2
1
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4
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2
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3
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4
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1
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2
1
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4
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3
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4
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1
1
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712866
Guirimand
Spatial organization of the vi ...
Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
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1
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3
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1
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3
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1
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1
1
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1
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1
1
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3
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1
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1
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1
1
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1
1
-
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-
676372
Magnotta
Identification of a low vindol ...
Catharanthus roseus
Phytochemistry
67
1758-1764
2006
-
-
-
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
2
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3
-
1
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1
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1
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1
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2
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2
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3
-
1
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1
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670558
Murata
localization of tabersonine 16 ...
Catharanthus roseus
Plant J.
44
581-594
2005
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1
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2
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3
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4
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2
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1
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1
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1
1
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2
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4
-
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2
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1
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438775
Schroder
Light-induced cytochrome P450- ...
Catharanthus roseus
FEBS Lett.
458
97-102
1999
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1
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1
1
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3
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1
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1
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1
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2
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438776
St-Pierre
A cytochrome P-450 monooxygena ...
Catharanthus roseus
Plant Physiol.
109
131-139
1995
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6
2
1
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1
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3
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4
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2
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1
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2
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6
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2
1
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1
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4
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2
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1
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2
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