BRENDA - Enzyme Database show
show all sequences of 1.14.14.103

Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus

Guirimand, G.; Guihur, A.; Poutrain, P.; Hericourt, F.; Mahroug, S.; St-Pierre, B.; Burlat, V.; Courdavault, V.; J. Plant Physiol. 168, 549-557 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determination and analysis
Catharanthus roseus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Catharanthus roseus
5783
-
endoplasmic reticulum membrane
T16H is anchored to the endoplasmic reticulum as a monomer via a putative 18-residue N-terminal helix
Catharanthus roseus
5789
-
additional information
vindoline biosynthesis pathway enzyme subcellular localization study, overview
Catharanthus roseus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tabersonine + NADPH + H+ + O2
Catharanthus roseus
-
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Catharanthus roseus
C0KYN4
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tabersonine + NADPH + H+ + O2
-
712866
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
-
Catharanthus roseus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Catharanthus roseus
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determination and analysis
Catharanthus roseus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Catharanthus roseus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Catharanthus roseus
5783
-
endoplasmic reticulum membrane
T16H is anchored to the endoplasmic reticulum as a monomer via a putative 18-residue N-terminal helix
Catharanthus roseus
5789
-
additional information
vindoline biosynthesis pathway enzyme subcellular localization study, overview
Catharanthus roseus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tabersonine + NADPH + H+ + O2
Catharanthus roseus
-
16-hydroxytabersonine + NADP+ + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Catharanthus roseus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tabersonine + NADPH + H+ + O2
-
712866
Catharanthus roseus
16-hydroxytabersonine + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
-
Catharanthus roseus
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the first step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. Absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview
Catharanthus roseus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes the first step in the biosynthesis of vindoline, which constitutes the main terpenoid indole alkaloid accumulated in leaves of Catharanthus roseus, spatial organization of the vindoline biosynthetic pathway, overview. Absence of metabolic channeling in the vindoline biosynthetic pathway. Importance of inter- and intracellular translocations of intermediates during the vindoline biosynthesis and potential regulatory role, overview
Catharanthus roseus
Other publictions for EC 1.14.14.103
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743890
Liu
Enhancement of vindoline and ...
Catharanthus roseus
World J. Microbiol. Biotechnol.
30
175-180
2014
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1
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2
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2
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1
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2
-
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-
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1
1
1
1
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728525
Besseau
A pair of tabersonine 16-hydro ...
Catharanthus roseus
Plant Physiol.
163
1792-1803
2013
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-
1
-
-
-
-
2
1
-
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4
-
2
-
-
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3
-
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4
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1
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2
1
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4
-
-
-
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3
-
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4
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712866
Guirimand
Spatial organization of the vi ...
Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
-
-
1
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-
-
-
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3
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1
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3
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1
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1
1
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1
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1
1
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3
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1
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1
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1
1
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-
-
-
-
-
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1
1
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676372
Magnotta
Identification of a low vindol ...
Catharanthus roseus
Phytochemistry
67
1758-1764
2006
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-
-
-
-
-
-
-
-
-
-
2
-
1
-
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2
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3
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1
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1
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1
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1
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2
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2
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3
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1
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1
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670558
Murata
localization of tabersonine 16 ...
Catharanthus roseus
Plant J.
44
581-594
2005
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1
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2
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3
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4
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2
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1
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1
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1
1
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2
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4
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2
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1
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438775
Schroder
Light-induced cytochrome P450- ...
Catharanthus roseus
FEBS Lett.
458
97-102
1999
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1
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1
1
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3
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2
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1
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1
1
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1
1
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2
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438776
St-Pierre
A cytochrome P-450 monooxygena ...
Catharanthus roseus
Plant Physiol.
109
131-139
1995
-
-
-
-
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6
2
1
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1
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3
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4
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2
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1
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2
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6
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2
1
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1
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4
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2
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1
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2
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