BRENDA - Enzyme Database show
show all sequences of 1.14.14.104

Enzymic biosynthesis of vomilenine, a key intermediate of the ajmaline pathway, catalyzed by a novel cytochrome P450-dependent enzyme from plant cell cultures of Rauwolfia serpentina

Falkenhagen, H.; Stoeckigt, J.; Z. Naturforsch. C 50, 45-53 (1995)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CO
-
Rauvolfia serpentina
cytochrome c
-
Rauvolfia serpentina
ketoconazole
-
Rauvolfia serpentina
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
26
-
vinorine
-
Rauvolfia serpentina
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
vinorine + [reduced NADPH-hemoprotein reductase] + O2
Rauvolfia serpentina
enzyme is involved in the biosynthesis of the alkaloid ajmaline
vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Rauvolfia serpentina
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rauvolfia serpentina
-
-
-
Storage Stability
Storage Stability
Organism
-28°C, 20% sucrose, no loss of activity
Rauvolfia serpentina
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
vinorine + [reduced NADPH-hemoprotein reductase] + O2
-
440250
Rauvolfia serpentina
vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O
-
440250
Rauvolfia serpentina
?
vinorine + [reduced NADPH-hemoprotein reductase] + O2
enzyme is involved in the biosynthesis of the alkaloid ajmaline
440250
Rauvolfia serpentina
vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O
-
440250
Rauvolfia serpentina
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Rauvolfia serpentina
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Rauvolfia serpentina
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Rauvolfia serpentina
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Rauvolfia serpentina
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CO
-
Rauvolfia serpentina
cytochrome c
-
Rauvolfia serpentina
ketoconazole
-
Rauvolfia serpentina
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
26
-
vinorine
-
Rauvolfia serpentina
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
vinorine + [reduced NADPH-hemoprotein reductase] + O2
Rauvolfia serpentina
enzyme is involved in the biosynthesis of the alkaloid ajmaline
vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O
-
Rauvolfia serpentina
?
Storage Stability (protein specific)
Storage Stability
Organism
-28°C, 20% sucrose, no loss of activity
Rauvolfia serpentina
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
vinorine + [reduced NADPH-hemoprotein reductase] + O2
-
440250
Rauvolfia serpentina
vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O
-
440250
Rauvolfia serpentina
?
vinorine + [reduced NADPH-hemoprotein reductase] + O2
enzyme is involved in the biosynthesis of the alkaloid ajmaline
440250
Rauvolfia serpentina
vomilenine + [oxidized NADPH-hemoprotein reductase] + H2O
-
440250
Rauvolfia serpentina
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Rauvolfia serpentina
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
-
Rauvolfia serpentina
Other publictions for EC 1.14.14.104
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744036
Dang
Dual catalytic activity of a ...
Rauvolfia serpentina
Angew. Chem. Int. Ed. Engl.
56
9440-9444
2017
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1
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2
1
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2
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4
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1
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1
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2
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1
2
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2
1
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2
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4
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1
-
-
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1
-
-
-
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3
3
-
-
-
440250
Falkenhagen
-
Enzymic biosynthesis of vomile ...
Rauvolfia serpentina
Z. Naturforsch. C
50
45-53
1995
-
-
-
-
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3
1
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-
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1
-
1
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-
-
-
-
-
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1
2
-
1
-
-
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1
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1
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-
1
-
-
-
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3
-
1
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-
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1
-
-
-
-
-
-
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1
2
-
1
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1
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