BRENDA - Enzyme Database show
show all sequences of 1.14.14.105

Molecular cloning of a cytochrome P450 taxane 10beta-hydroxylase cDNA from Taxus and functional expression in yeast

Schoendorf, A.; Rithner, C.D.; Williams, R.M.; Croteau, R.B.; Proc. Natl. Acad. Sci. USA 98, 1501-1506 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Taxus sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
in vivo and in vitro assay in yeast for recombinant enzyme
Taxus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Taxus sp.
catalyzes early step in taxol biosynthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus sp.
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Taxus sp.
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
catalyzes early step in taxol biosynthesis
438774
Taxus sp.
?
-
-
-
-
taxa-4(20),11-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
438774
Taxus sp.
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Taxus sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Taxus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Taxus sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
in vivo and in vitro assay in yeast for recombinant enzyme
Taxus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Taxus sp.
catalyzes early step in taxol biosynthesis
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell culture
-
Taxus sp.
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
catalyzes early step in taxol biosynthesis
438774
Taxus sp.
?
-
-
-
-
taxa-4(20),11-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
438774
Taxus sp.
10beta-hydroxytaxa-4(20),11-dien-5alpha-yl acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.105
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672704
DeJong
Genetic engineering of taxol b ...
Taxus sp.
Biotechnol. Bioeng.
93
212-224
2006
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
675630
Kai
-
Isolation and expression profi ...
Taxus x media
J. Plant Biochem. Biotechnol.
15
1-5
2006
1
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
672699
Jennewein
Coexpression in yeast of Taxus ...
Taxus cuspidata
Biotechnol. Bioeng.
89
588-598
2005
-
-
1
-
1
-
-
-
2
1
-
1
-
5
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
1
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
438774
Schoendorf
Molecular cloning of a cytochr ...
Taxus sp.
Proc. Natl. Acad. Sci. USA
98
1501-1506
2001
-
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
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-
-
-
-
-
-
1
-
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-
-
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
2
-
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