BRENDA - Enzyme Database show
show all sequences of 1.14.14.105

Genetic engineering of taxol biosynthetic genes in Saccharomyces cerevisiae

DeJong, J.H.; Liu, Y.; Bollon, A.P.; Long, R.M.; Jennewein, S.; Williams, D.; Croteau, R.B.; Biotechnol. Bioeng. 93, 212-224 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression of the FLAG-tagged enzyme in Saccharomyces cerevisiae YPH499 cells, expression of the enzymes from and reconstruction of the taxol biosynthesis pathway 5 early steps by expression of the participating enzymes in yeast, overview
Taxus sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
Taxus sp.
step in taxol biosynthesis, metabolite analysis and pathway overview
taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus sp.
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant FLAG-tagged enzyme from Saccharomyces cerevisiae YPH499 cells
Taxus sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
672704
Taxus sp.
taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
product identification and quantification by HPLC and GC-MS
-
-
-
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
step in taxol biosynthesis, metabolite analysis and pathway overview
672704
Taxus sp.
taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
31
-
assay at
Taxus sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Taxus sp.
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Taxus sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
expression of the FLAG-tagged enzyme in Saccharomyces cerevisiae YPH499 cells, expression of the enzymes from and reconstruction of the taxol biosynthesis pathway 5 early steps by expression of the participating enzymes in yeast, overview
Taxus sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Taxus sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
Taxus sp.
step in taxol biosynthesis, metabolite analysis and pathway overview
taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant FLAG-tagged enzyme from Saccharomyces cerevisiae YPH499 cells
Taxus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
672704
Taxus sp.
taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
product identification and quantification by HPLC and GC-MS
-
-
-
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
step in taxol biosynthesis, metabolite analysis and pathway overview
672704
Taxus sp.
taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
31
-
assay at
Taxus sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Taxus sp.
Other publictions for EC 1.14.14.105
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672704
DeJong
Genetic engineering of taxol b ...
Taxus sp.
Biotechnol. Bioeng.
93
212-224
2006
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
675630
Kai
-
Isolation and expression profi ...
Taxus x media
J. Plant Biochem. Biotechnol.
15
1-5
2006
1
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
1
-
1
-
1
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
3
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
672699
Jennewein
Coexpression in yeast of Taxus ...
Taxus cuspidata
Biotechnol. Bioeng.
89
588-598
2005
-
-
1
-
1
-
-
-
2
1
-
1
-
5
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
1
3
-
1
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
438774
Schoendorf
Molecular cloning of a cytochr ...
Taxus sp.
Proc. Natl. Acad. Sci. USA
98
1501-1506
2001
-
-
1
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-