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Literature summary for 1.14.14.105 extracted from

  • Kai, G.; Jiang, J.; Zhao, D.; Zhao, L.; Zhang, L.; Li, Z.; Guo, B.; Sun, X.; Miao, Z.; Tang, K.
    Isolation and expression profile analysis of a new cDNA encoding 5-alpha-taxadienol-10-beta-hydroxylase from Taxus media (2006), J. Plant Biochem. Biotechnol., 15, 1-5.
No PubMed abstract available
Search for more literature for 1.14.14.105

Activating Compound

Activating Compound Comment Organism Structure
additional information elicitors methyl jasmonate, AgNO3, and ammonium ceric sulfate all induce expression of the enzyme Taxus x media

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Taxus x media

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 x * 57000, about, amino acid sequence calculation Taxus x media

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 Taxus x media second P450-dependent step in taxol biosynthesis, regulation mechanism involving elicitors taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Taxus x media
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf young, strong expression level Taxus x media
-
additional information tissue expression pattern, no expressionin fruits, overview Taxus x media
-
stem weak expression level Taxus x media
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
 Taxus x media taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
taxa-4(20),11(12)-dien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2 second P450-dependent step in taxol biosynthesis, regulation mechanism involving elicitors Taxus x media taxa-4(20),11(12)-dien-5alpha-yl acetyl-10beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 57000, about, amino acid sequence calculation Taxus x media

Synonyms

Synonyms Comment Organism
5-alpha-taxadienol-10-beta-hydroxylase
-
 Taxus x media
Tm10BH
-
 Taxus x media

Cofactor

Cofactor Comment Organism Structure
cytochrome P-450
-
 Taxus x media

pI Value

Organism Comment pI Value Maximum pI Value
Taxus x media amino acid sequence calculation
-
 8.9