BRENDA - Enzyme Database show
show all sequences of 1.14.14.106

Coexpression in yeast of Taxus cytochrome P450 reductase with cytochrome P450 oxygenases involved in taxol biosynthesis

Jennewein, S.; Park, H.; DeJong, J.M.; Long, R.M.; Bollon, A.P.; Croteau, R.B.; Biotechnol. Bioeng. 89, 588-598 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
functional co-expression with taxoid 10beta-hydroxylase in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli
Taxus cuspidata
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Taxus cuspidata
5783
-
microsome
-
Taxus cuspidata
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
heme iron
Taxus cuspidata
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
Taxus cuspidata
the enzyme is involved in the taxol biosynthetic pathway, overview
taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Taxus cuspidata
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Taxus cuspidata
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in the taxol biosynthetic pathway, overview
672699
Taxus cuspidata
taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
672699
Taxus cuspidata
taxa-4(20),11-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
assay at
Taxus cuspidata
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Taxus cuspidata
Cloned(Commentary) (protein specific)
Commentary
Organism
functional co-expression with taxoid 10beta-hydroxylase in Saccharomyces cerevisiae strain YPH499, subcloning in Escherichia coli
Taxus cuspidata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
-
Taxus cuspidata
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Taxus cuspidata
5783
-
microsome
-
Taxus cuspidata
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
heme iron
Taxus cuspidata
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
Taxus cuspidata
the enzyme is involved in the taxol biosynthetic pathway, overview
taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Taxus cuspidata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in the taxol biosynthetic pathway, overview
672699
Taxus cuspidata
taxa-4(20),11(12)-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
taxadien-5alpha-yl acetate + [reduced NADPH-hemoprotein reductase] + O2
-
672699
Taxus cuspidata
taxa-4(20),11-dien-5alpha-yl-acetyl-13alpha-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
assay at
Taxus cuspidata
Other publictions for EC 1.14.14.106
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
672704
DeJong
Genetic engineering of taxol b ...
Taxus sp.
Biotechnol. Bioeng.
93
212-224
2006
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1
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1
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1
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1
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1
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1
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1
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1
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2
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1
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1
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-
672699
Jennewein
Coexpression in yeast of Taxus ...
Taxus cuspidata
Biotechnol. Bioeng.
89
588-598
2005
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-
1
-
-
-
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-
2
1
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1
-
1
-
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1
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2
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1
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1
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1
1
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2
1
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1
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1
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2
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1
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440248
Wheeler
Taxol biosynthesis: differenti ...
Taxus cuspidata
Arch. Biochem. Biophys.
390
265-278
2001
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2
1
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1
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1
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1
1
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2
1
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1
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2
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1
1
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440249
Jennewein
Taxol biosynthesis: taxane 13a ...
Taxus cuspidata
Proc. Natl. Acad. Sci. USA
98
13595-13600
2001
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1
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2
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1
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1
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1
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1
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2
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1
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1
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