BRENDA - Enzyme Database show
show all sequences of 1.14.14.107

The CYP88A cytochrome P450, ent-kaurenoic acid oxidase, catalyzes three steps of the gibberellin biosynthesis pathway

Helliwell, C.A.; Chandler, P.M.; Poole, A.; Dennis, E.S.; Peacock, W.J.; Proc. Natl. Acad. Sci. USA 98, 2065-2070 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in yeast
Hordeum vulgare
expression in yeast
Zea mays
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Hordeum vulgare
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Hordeum vulgare
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
Hordeum vulgare
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Hordeum vulgare
Q9AXH9
dwarf mutant of barley that accumulates ent-keurenoic acid in developing grains
-
Zea mays
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Hordeum vulgare
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Zea mays
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Arabidopsis thaliana
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Hordeum vulgare
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Zea mays
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Arabidopsis thaliana
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Hordeum vulgare
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Zea mays
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Arabidopsis thaliana
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P450 protein
Hordeum vulgare
cytochrome P450
cytochrome P450 protein
Zea mays
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in yeast
Hordeum vulgare
expression in yeast
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P450 protein
Hordeum vulgare
cytochrome P450
cytochrome P450 protein
Zea mays
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Hordeum vulgare
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Hordeum vulgare
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
Hordeum vulgare
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
Zea mays
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
Arabidopsis thaliana
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Hordeum vulgare
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Zea mays
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-7alpha-hydroxykaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Arabidopsis thaliana
gibberellin A12 aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Hordeum vulgare
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Zea mays
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
ent-kaur-16-en-19-oate + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Arabidopsis thaliana
ent-7alpha-hydroxykaur-16-en-19-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Hordeum vulgare
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Zea mays
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
gibberellin A12 aldehyde + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes the three steps of the gibberellin biosynthetic pathway from ent-kaurenoic acid to gibberellin A12
440385
Arabidopsis thaliana
gibberellin A12 + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.107
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745468
Cho
OsWOX3A is involved in negati ...
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746147
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Role of reactive oxygen speci ...
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Plant Signal. Behav.
11
e1180492
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745483
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Characterisation of two genes ...
Malus domestica
J. Hortic. Sci. Biotechnol.
89
329-337
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746078
Regnault
The gibberellin biosynthetic ...
Arabidopsis thaliana
Plant J.
80
462-474
2014
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15
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727594
Yang
Molecular evolution of the ent ...
Chikusichloa aquatica, Ehrharta erecta, Leersia tisserantii, Luziola fluitans, Oryza brachyantha, Oryza meridionalis, Oryza meyeriana var. granulata, Oryza officinalis, Oryza punctata, Oryza sativa, Rhynchoryza subulata
Genet. Mol. Biol.
35
448-454
2012
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700131
Khlestkina
-
Ent-kaurenoic acid oxidase gen ...
Aegilops tauschii, Triticum aestivum, Triticum urartu
Mol. Breed.
25
251-258
2009
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688097
Sawada
Germination of photoblastic le ...
Lactuca sativa
J. Exp. Bot.
59
3383-3393
2008
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660237
Sakamoto
An overview of gibberellin met ...
Oryza sativa
Plant Physiol.
134
1642-1653
2004
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440386
Davidson
The pea gene NA encodes ent-ka ...
Pisum sativum
Plant Physiol.
131
335-344
2003
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440385
Helliwell
The CYP88A cytochrome P450, en ...
Arabidopsis thaliana, Hordeum vulgare, Zea mays
Proc. Natl. Acad. Sci. USA
98
2065-2070
2001
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9
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