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Literature summary for 1.14.14.108 extracted from

  • Iwaki, H.; Grosse, S.; Bergeron, H.; Leisch, H.; Morley, K.; Hasegawa, Y.; Lau, P.C.
    Camphor pathway redux functional recombinant expression of 2,5- and 3,6-diketocamphane monooxygenases of Pseudomonas putida ATCC 17453 with their cognate flavin reductase catalyzing Baeyer-Villiger reactions (2013), Appl. Environ. Microbiol., 79, 3282-3293 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.108

Cloned(Commentary)

Cloned (Comment) Organism
to enable functional assessment of the two-component monooxygenase system in Baeyer-Villiger oxidations, a recombinant plasmid expressing Fred (FMN reductase (NADH)) in tandem with 2,5-DKCMO-encoding gene in Escherichia coli is constructed Pseudomonas putida
to enable functional assessment of the two-component monooxygenase system in Baeyer-Villiger oxidations, recombinant plasmids expressing Fred (FMN reductase (NADH)) in tandem with the respective 2,5-DKCMO- and 3,6-DKCMO-encoding genes in Escherichia coli are constructed Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40574
-
 calculated from sequence Pseudomonas putida
40702
-
 calculated from sequence Pseudomonas putida
41000
-
 SDS-PAGE Pseudomonas putida
60000
-
 gel filtration Pseudomonas putida
64000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(+)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida the enzyme is involved in the degradation of (-)-camphor (+)-5-oxo-1,2-campholide + FMN + H2O
-
 ?
(+)-bornane-2,5-dione + FMNH2 + O2 Pseudomonas putida ATCC 17453 the enzyme is involved in the degradation of (-)-camphor (+)-5-oxo-1,2-campholide + FMN + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida M5AWY0
-
-
Pseudomonas putida Q6STM1
-
-
Pseudomonas putida ATCC 17453 M5AWY0
-
-
Pseudomonas putida ATCC 17453 Q6STM1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(+)-bornane-2,5-dione + FMNH2 + O2 the enzyme is involved in the degradation of (-)-camphor Pseudomonas putida (+)-5-oxo-1,2-campholide + FMN + H2O
-
 ?
(+)-bornane-2,5-dione + FMNH2 + O2 the enzyme requires a dedicated NADH-FMN reductase (FMN reductase (NADH)), FMN-dependent two-component monooxygenase system Pseudomonas putida (+)-5-oxo-1,2-campholide + FMN + H2O
-
 ?
(+)-bornane-2,5-dione + FMNH2 + O2 the enzyme is involved in the degradation of (-)-camphor Pseudomonas putida ATCC 17453 (+)-5-oxo-1,2-campholide + FMN + H2O
-
 ?
(+)-bornane-2,5-dione + FMNH2 + O2 the enzyme requires a dedicated NADH-FMN reductase (FMN reductase (NADH)), FMN-dependent two-component monooxygenase system Pseudomonas putida ATCC 17453 (+)-5-oxo-1,2-campholide + FMN + H2O
-
 ?
(+)-camphor + FMNH2 + O2 conversion to lactone, no concersion of (-)-camphor Pseudomonas putida ?
-
 ?
(+)-camphor + FMNH2 + O2 conversion to lactone, no concersion of (-)-camphor Pseudomonas putida ATCC 17453 ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41000, SDS-PAGE Pseudomonas putida

Synonyms

Synonyms Comment Organism
2,5-diketocamphane monooxygenase
-
 Pseudomonas putida
2,5-DKCMO-1
-
 Pseudomonas putida
2,5-DKCMO-2
-
 Pseudomonas putida
camE25-1 gene name, two isofunctional 2,5-DKCMO genes (camE25-1 for 2,5-DKCMO-1 and camE25-2 for 2,5-DKCMO-2) Pseudomonas putida
camE25-2 gene name, two isofunctional 2,5-DKCMO genes (camE25-1 for 2,5-DKCMO-1 and camE25-2 for 2,5-DKCMO-2) Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
 Tm-value Pseudomonas putida
63
-
 Tm-value Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Pseudomonas putida
8
-
-
 Pseudomonas putida

General Information

General Information Comment Organism
metabolism the enzyme is involved in the degradation of (-)-camphor Pseudomonas putida