BRENDA - Enzyme Database show
show all sequences of 1.14.14.110

Maize microsomal benzoxazinone N-monooxygenase

Bailey, B.A.; Larson, R.L.; Plant Physiol. 95, 792-796 (1991)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
49% enhancement of activity at 5 mM
Zea mays
additional information
not affected by 5 mM diethyldithiocarbamate
Zea mays
General Stability
General Stability
Organism
extraction of microsomes in the absence of dithioerythritol results in total loss of activity, but exclusion of dithioerythritol from the assay buffer has no effect on enzyme activity
Zea mays
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
5 mM inhibits activity by 57%
Zea mays
Ca2+
5 mM Ca2+ inhibits activity by 69%
Zea mays
carbon monoxide
the effect of carbon monoxide can be reversed by exposing the enzyme to 450 nanometer light during the incubation period
Zea mays
cytochrome c
-
Zea mays
Fe2+
5 mM Fe2+ inhibits activity by 64%
Zea mays
Hg2+
complete inhibition at 5 mM
Zea mays
Mg2+
5 mM Mg2+ inhibits activity by 45%
Zea mays
Mn2+
complete inhibition at 5 mM
Zea mays
N-ethylmaleimide
5 mM inhibits activity by 97%
Zea mays
N2
the effect of nitrogen can be reversed by exposing the enzyme to air
Zea mays
NADP+
-
Zea mays
NaN3
5 mM NaN3 inhibits activity by 16%
Zea mays
p-chloromercuribenzoate
5 mM inhibits activity by 58%
Zea mays
Zn2+
complete inhibition at 5 mM
Zea mays
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
in 50 mM HEPES, pH 8.5, at 35°C
Zea mays
0.013
-
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one
in 50 mM HEPES, pH 8.5, at 35°C
Zea mays
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Zea mays
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zea mays
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
seedling
-
Zea mays
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [reduced NADPH-hemoprotein reductase] + O2
-
723478
Zea mays
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with NADH and FAD
723478
Zea mays
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Zea mays
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
heating the enzyme for 10 min at 100°C in a boiling water bath results in a total loss of activity
Zea mays
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Zea mays
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P-450
dependent on
Zea mays
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
49% enhancement of activity at 5 mM
Zea mays
additional information
not affected by 5 mM diethyldithiocarbamate
Zea mays
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P-450
dependent on
Zea mays
General Stability (protein specific)
General Stability
Organism
extraction of microsomes in the absence of dithioerythritol results in total loss of activity, but exclusion of dithioerythritol from the assay buffer has no effect on enzyme activity
Zea mays
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,2'-dipyridyl
5 mM inhibits activity by 57%
Zea mays
Ca2+
5 mM Ca2+ inhibits activity by 69%
Zea mays
carbon monoxide
the effect of carbon monoxide can be reversed by exposing the enzyme to 450 nanometer light during the incubation period
Zea mays
cytochrome c
-
Zea mays
Fe2+
5 mM Fe2+ inhibits activity by 64%
Zea mays
Hg2+
complete inhibition at 5 mM
Zea mays
Mg2+
5 mM Mg2+ inhibits activity by 45%
Zea mays
Mn2+
complete inhibition at 5 mM
Zea mays
N-ethylmaleimide
5 mM inhibits activity by 97%
Zea mays
N2
the effect of nitrogen can be reversed by exposing the enzyme to air
Zea mays
NADP+
-
Zea mays
NaN3
5 mM NaN3 inhibits activity by 16%
Zea mays
p-chloromercuribenzoate
5 mM inhibits activity by 58%
Zea mays
Zn2+
complete inhibition at 5 mM
Zea mays
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
NADPH
in 50 mM HEPES, pH 8.5, at 35°C
Zea mays
0.013
-
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one
in 50 mM HEPES, pH 8.5, at 35°C
Zea mays
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Zea mays
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
seedling
-
Zea mays
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxy-2H-1,4-benzoxazin-3(4H)-one + [reduced NADPH-hemoprotein reductase] + O2
-
723478
Zea mays
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
no activity with NADH and FAD
723478
Zea mays
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Zea mays
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
100
-
heating the enzyme for 10 min at 100°C in a boiling water bath results in a total loss of activity
Zea mays
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
-
Zea mays
Other publictions for EC 1.14.14.110
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723452
Persans
Characterization of maize cyto ...
Zea mays
Plant Physiol.
125
1126-1138
2001
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
723355
Glawischnig
Cytochrome P450 monooxygenases ...
Zea mays
Phytochemistry
50
925-930
1999
-
-
1
-
-
-
-
2
1
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
723478
Bailey
Maize microsomal benzoxazinone ...
Zea mays
Plant Physiol.
95
792-796
1991
2
-
-
-
-
1
14
2
1
-
-
-
-
2
-
-
-
-
-
1
-
-
2
-
1
-
1
-
1
-
-
1
-
-
-
2
-
-
1
-
-
1
-
14
-
2
1
-
-
-
-
-
-
-
-
1
-
-
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-