BRENDA - Enzyme Database show
show all sequences of 1.14.14.113

Efficient functional analysis system for cyanobacterial or plant cytochromes P450 involved in sesquiterpene biosynthesis

Harada, H.; Shindo, K.; Iki, K.; Teraoka, A.; Okamoto, S.; Yu, F.; Hattan, J.; Utsumi, R.; Misawa, N.; Appl. Microbiol. Biotechnol. 90, 467-476 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
CYP71BA1 expression in Escherichia coli. Co-expression with germacrene A hydroxylase P450NSor CYP110C1 from Nostoc sp. PCC 7120 and the Arabidopsis thaliana ATR2 as well as two corresponding enzymes derived from Zingiber officinale, named ZoRED1 and ZoRED2 from one singel expression plasmid, construction, overview. The co-expression enhances CYP71BA1 expression levels by 1.5-2fold comapred to single heterologous expression
Zingiber zerumbet
Engineering
Amino acid exchange
Commentary
Organism
additional information
reconstruction of the isoprenoid biosynthetic pathway in Escherichia coli, overview
Zingiber zerumbet
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
cytochrome P450 enzyme
Zingiber zerumbet
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
Zingiber zerumbet
-
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zingiber zerumbet
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
-
717135
Zingiber zerumbet
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zingiber zerumbet
Cloned(Commentary) (protein specific)
Commentary
Organism
CYP71BA1 expression in Escherichia coli. Co-expression with germacrene A hydroxylase P450NSor CYP110C1 from Nostoc sp. PCC 7120 and the Arabidopsis thaliana ATR2 as well as two corresponding enzymes derived from Zingiber officinale, named ZoRED1 and ZoRED2 from one singel expression plasmid, construction, overview. The co-expression enhances CYP71BA1 expression levels by 1.5-2fold comapred to single heterologous expression
Zingiber zerumbet
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zingiber zerumbet
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
reconstruction of the isoprenoid biosynthetic pathway in Escherichia coli, overview
Zingiber zerumbet
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
cytochrome P450 enzyme
Zingiber zerumbet
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
Zingiber zerumbet
-
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
-
717135
Zingiber zerumbet
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the cytochrome P450 monooxygenases
Zingiber zerumbet
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the cytochrome P450 monooxygenases
Zingiber zerumbet
Other publictions for EC 1.14.14.113
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717135
Harada
Efficient functional analysis ...
Zingiber zerumbet
Appl. Microbiol. Biotechnol.
90
467-476
2011
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717468
Yu
Zingiber zerumbet CYP71BA1 cat ...
Zingiber zerumbet
Cell. Mol. Life Sci.
68
1033-1040
2011
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717593
Okamoto
A short-chain dehydrogenase in ...
Zingiber zerumbet
FEBS J.
278
2892-2900
2011
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