BRENDA - Enzyme Database show
show all sequences of 1.14.14.113

Zingiber zerumbet CYP71BA1 catalyzes the conversion of ?-humulene to 8-hydroxy-alpha-humulene in zerumbone biosynthesis

Yu, F.; Okamoto, S.; Harada, H.; Yamasaki, K.; Misawa, N.; Utsumi, R.; Cell. Mol. Life Sci. 68, 1033-1040 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
functional co-expression of CYP71BA1 with Arabidopsis NADPH-dependent P450 reductase in Saccharomyces cerevisiae strain WAT11 microsomes, additional cloning of Arabidopsis thaliana ATR2 and Zingiber zerumbet ZSS1 encoding an alpha-humulene synthase
Zingiber zerumbet
Engineering
Amino acid exchange
Commentary
Organism
additional information
metabolic engineering: functional co-expression of a gene cluster encoding four enzymes of the mevalonate pathway, i.e. mevalonate kinase, phosphomevalonate kinase, mevalonate diphosphate decarboxylase, and isopentenyl diphosphate isomerase, with CYP71BA1 and ZSS1, encoding alpha-humulene synthase, in Escherichia coli leading to the production of 10-hydroxy-alpha-humulene in the presence of mevalonate, overview
Zingiber zerumbet
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
cytochrome P450 enzyme
Zingiber zerumbet
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
Zingiber zerumbet
-
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Zingiber zerumbet
E3W9C4
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
rhizome
CYP71BA1 transcripts are detected almost exclusively in rhizomes, expression pattern of ZSS1 transcripts during rhizome development
Zingiber zerumbet
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
-
717468
Zingiber zerumbet
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
CYP71BA1 is a regiospecific cytochrome P450 enzyme
717468
Zingiber zerumbet
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
GC-MS product analysis
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Zingiber zerumbet
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Zingiber zerumbet
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zingiber zerumbet
Cloned(Commentary) (protein specific)
Commentary
Organism
functional co-expression of CYP71BA1 with Arabidopsis NADPH-dependent P450 reductase in Saccharomyces cerevisiae strain WAT11 microsomes, additional cloning of Arabidopsis thaliana ATR2 and Zingiber zerumbet ZSS1 encoding an alpha-humulene synthase
Zingiber zerumbet
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Zingiber zerumbet
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
metabolic engineering: functional co-expression of a gene cluster encoding four enzymes of the mevalonate pathway, i.e. mevalonate kinase, phosphomevalonate kinase, mevalonate diphosphate decarboxylase, and isopentenyl diphosphate isomerase, with CYP71BA1 and ZSS1, encoding alpha-humulene synthase, in Escherichia coli leading to the production of 10-hydroxy-alpha-humulene in the presence of mevalonate, overview
Zingiber zerumbet
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
cytochrome P450 enzyme
Zingiber zerumbet
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
Zingiber zerumbet
-
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
rhizome
CYP71BA1 transcripts are detected almost exclusively in rhizomes, expression pattern of ZSS1 transcripts during rhizome development
Zingiber zerumbet
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
-
717468
Zingiber zerumbet
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
alpha-humulene + O2 + [reduced NADPH-hemoprotein reductase]
CYP71BA1 is a regiospecific cytochrome P450 enzyme
717468
Zingiber zerumbet
0-hydroxy-alpha-humulene + [oxidized NADPH-hemoprotein reductase] + H2O
GC-MS product analysis
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Zingiber zerumbet
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Zingiber zerumbet
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the cytochrome P450 monooxygenases
Zingiber zerumbet
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the cytochrome P450 monooxygenases
Zingiber zerumbet
Other publictions for EC 1.14.14.113
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
717135
Harada
Efficient functional analysis ...
Zingiber zerumbet
Appl. Microbiol. Biotechnol.
90
467-476
2011
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717468
Yu
Zingiber zerumbet CYP71BA1 cat ...
Zingiber zerumbet
Cell. Mol. Life Sci.
68
1033-1040
2011
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717593
Okamoto
A short-chain dehydrogenase in ...
Zingiber zerumbet
FEBS J.
278
2892-2900
2011
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