BRENDA - Enzyme Database show
show all sequences of 1.14.14.114

Molecular cloning of an aldehyde dehydrogenase implicated in artemisinin biosynthesis in Artemisia annua

Teoh, K.; Polichuk, D.; Reed, D.; Covello, P.; Botany 87, 635-642 (2009)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Artemisia annua
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
artemisinic aldehyde
pH 8.5, 30C
Artemisia annua
0.0088
-
dihydroartemisinic aldehyde
pH 8.5, 30C
Artemisia annua
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53800
-
x * 53800, calculated
Artemisia annua
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Artemisia annua
-
-
-
Artemisia annua
C5I9X1
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
trichome
glandular secretory trichome
Artemisia annua
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-nonenal + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
? + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
amorpha-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
artemisinic alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
artemisinic alcohol + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
artemisinic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
artemisinic aldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
artemisinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
dihydroartemisinic alcohol + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
dihydroartemisinic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
dihydroartemisinic alcohol + [reduced NADPH-hemoprotein reductase] + O2
about 50% of the rate with artemisinic alcohol
717445
Artemisia annua
dihydroartemisinic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
dihydroartemisinic aldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
dihydroartemisinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
additional information
no substrate: dihydroartemisinic aldehyde
717445
Artemisia annua
?
-
-
-
-
additional information
no substrates: Sinapaldehyde, 2-phenylpropanal
717445
Artemisia annua
?
-
-
-
-
octanal + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
? + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 53800, calculated
Artemisia annua
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.53
-
artemisinic aldehyde
pH 8.5, 30C
Artemisia annua
7.74
-
dihydroartemisinic aldehyde
pH 8.5, 30C
Artemisia annua
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
substrate dihydroartemisinic aldehyde
Artemisia annua
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Artemisia annua
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0026
-
artemisinic aldehyde
pH 8.5, 30C
Artemisia annua
0.0088
-
dihydroartemisinic aldehyde
pH 8.5, 30C
Artemisia annua
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
53800
-
x * 53800, calculated
Artemisia annua
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
trichome
glandular secretory trichome
Artemisia annua
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-nonenal + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
? + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
amorpha-4,11-diene + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
artemisinic alcohol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
artemisinic alcohol + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
artemisinic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
artemisinic aldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
artemisinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
dihydroartemisinic alcohol + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
dihydroartemisinic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
dihydroartemisinic alcohol + [reduced NADPH-hemoprotein reductase] + O2
about 50% of the rate with artemisinic alcohol
717445
Artemisia annua
dihydroartemisinic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
dihydroartemisinic aldehyde + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
dihydroartemisinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
additional information
no substrate: dihydroartemisinic aldehyde
717445
Artemisia annua
?
-
-
-
-
additional information
no substrates: Sinapaldehyde, 2-phenylpropanal
717445
Artemisia annua
?
-
-
-
-
octanal + [reduced NADPH-hemoprotein reductase] + O2
-
717445
Artemisia annua
? + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 53800, calculated
Artemisia annua
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.53
-
artemisinic aldehyde
pH 8.5, 30C
Artemisia annua
7.74
-
dihydroartemisinic aldehyde
pH 8.5, 30C
Artemisia annua
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
substrate dihydroartemisinic aldehyde
Artemisia annua
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
593
-
artemisinic aldehyde
pH 8.5, 30C
Artemisia annua
881
-
dihydroartemisinic aldehyde
pH 8.5, 30C
Artemisia annua
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
593
-
artemisinic aldehyde
pH 8.5, 30C
Artemisia annua
881
-
dihydroartemisinic aldehyde
pH 8.5, 30C
Artemisia annua
Other publictions for EC 1.14.14.114
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745821
Chen
Multienzyme biosynthesis of d ...
Artemisia annua
Molecules
22
1422-1433
2017
-
1
1
-
1
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
3
-
-
-
-
1
1
3
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
-
-
2
2
-
-
-
745905
Chen
Glandular trichome-specific W ...
Artemisia annua
New Phytol.
214
304-316
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
1
1
1
1
-
-
746043
Muangphrom
Functional analysis of amorph ...
Artemisia absinthium, Artemisia afra, Artemisia annua, Artemisia kurramensis
Plant Cell Physiol.
57
1678-1688
2016
-
-
3
-
-
-
-
-
-
-
-
4
-
9
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
8
-
-
-
-
-
3
8
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
4
-
-
-
-
-
-
-
-
-
-
8
8
-
-
-
746069
Zheng
-
The influence of endophytic P ...
Artemisia annua
Plant Growth Regul.
80
93-102
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
746309
Czechowski
Artemisia annua mutant impair ...
Artemisia annua
Proc. Natl. Acad. Sci. USA
113
15150-15155
2016
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
4
-
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
4
-
-
5
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
746035
Ji
Cloning and characterization ...
Artemisia annua
Plant Cell Physiol.
55
1592-1604
2014
-
-
1
-
-
-
-
-
-
-
-
4
-
3
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
716656
Alejos-Gonzalez
Characterization of developmen ...
Artemisia annua
Planta
234
685-697
2011
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
718205
Zhang
The production of artemisinin ...
Artemisia annua
Plant Biotechnol. J.
9
445-454
2011
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
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-
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-
-
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-
706125
Olsson
Localization of enzymes of art ...
Artemisia annua
Phytochemistry
70
1123-1128
2009
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
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-
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
717445
Teoh
-
Molecular cloning of an aldehy ...
Artemisia annua
Botany
87
635-642
2009
-
-
1
-
-
-
-
2
-
-
1
-
-
2
-
-
-
-
-
1
-
-
13
1
-
-
-
2
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
13
1
-
-
-
2
1
-
-
-
-
-
-
-
2
2
717601
Teoh
Artemisia annua L. (Asteraceae ...
Artemisia annua
FEBS Lett.
580
1411-1416
2006
-
-
1
-
-
-
-
-
-
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-
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4
-
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4
-
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4
-
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1
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4
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4
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