BRENDA - Enzyme Database show
show all sequences of 1.14.14.114

The influence of endophytic Penicillium oxalicum B4 on growth and artemisinin biosynthesis of in vitro propagated plantlets of Artemisia annua L.

Zheng, L.; Tian, H.; Yuan, Y.; Wang, J.; Plant Growth Regul. 80, 93-102 (2016)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase]
Artemisia annua
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Artemisia annua
Q1PS23
cv. CQF39
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
callus cannot produce artemisinin
Artemisia annua
-
seedling
regenerated plantlets
Artemisia annua
-
trichome
artemisinin production in Artemisia annua is localized in glandular trichomes on the surface of leaves, shoots and flowers
Artemisia annua
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase]
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
746069
Artemisia annua
artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Artemisia annua
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Artemisia annua
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Artemisia annua
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase]
Artemisia annua
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
callus cannot produce artemisinin
Artemisia annua
-
seedling
regenerated plantlets
Artemisia annua
-
trichome
artemisinin production in Artemisia annua is localized in glandular trichomes on the surface of leaves, shoots and flowers
Artemisia annua
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase]
overall reaction via artemsinic alcohol and artemsinic aldehyde, multistep process
746069
Artemisia annua
artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Artemisia annua
Expression
Organism
Commentary
Expression
Artemisia annua
endophyte Penicillium oxalicum strain B4 induces the enzyme and leads to enhanced growth and artemisinin content of host plant in an in vitro dual culture of endophyte with regenerated plantlets via generation of reactive oxygen species (ROS) including superoxide ions and H2O2
up
Expression (protein specific)
Organism
Commentary
Expression
Artemisia annua
endophyte Penicillium oxalicum strain B4 induces the enzyme and leads to enhanced growth and artemisinin content of host plant in an in vitro dual culture of endophyte with regenerated plantlets via generation of reactive oxygen species (ROS) including superoxide ions and H2O2
up
Other publictions for EC 1.14.14.114
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745821
Chen
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Artemisia annua
Molecules
22
1422-1433
2017
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1
1
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1
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4
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1
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1
1
3
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1
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4
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4
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1
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2
2
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-
745905
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Glandular trichome-specific W ...
Artemisia annua
New Phytol.
214
304-316
2017
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1
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4
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2
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1
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4
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1
1
1
1
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746043
Muangphrom
Functional analysis of amorph ...
Artemisia absinthium, Artemisia afra, Artemisia annua, Artemisia kurramensis
Plant Cell Physiol.
57
1678-1688
2016
-
-
3
-
-
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-
-
-
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4
-
9
-
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3
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4
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8
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3
8
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4
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3
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4
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8
8
-
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-
746069
Zheng
-
The influence of endophytic P ...
Artemisia annua
Plant Growth Regul.
80
93-102
2016
-
-
-
-
-
-
-
-
-
-
-
1
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1
-
-
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3
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1
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1
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1
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1
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1
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3
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1
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1
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1
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1
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746309
Czechowski
Artemisia annua mutant impair ...
Artemisia annua
Proc. Natl. Acad. Sci. USA
113
15150-15155
2016
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1
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4
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2
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4
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5
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2
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2
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1
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4
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4
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5
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3
3
-
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746035
Ji
Cloning and characterization ...
Artemisia annua
Plant Cell Physiol.
55
1592-1604
2014
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-
1
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4
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3
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1
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4
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1
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1
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4
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1
2
2
1
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-
716656
Alejos-Gonzalez
Characterization of developmen ...
Artemisia annua
Planta
234
685-697
2011
-
1
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2
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2
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1
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2
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718205
Zhang
The production of artemisinin ...
Artemisia annua
Plant Biotechnol. J.
9
445-454
2011
-
1
-
-
-
-
-
-
-
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3
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706125
Olsson
Localization of enzymes of art ...
Artemisia annua
Phytochemistry
70
1123-1128
2009
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1
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717445
Teoh
-
Molecular cloning of an aldehy ...
Artemisia annua
Botany
87
635-642
2009
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1
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2
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1
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2
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1
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13
1
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2
1
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1
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2
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1
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1
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13
1
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2
1
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2
2
717601
Teoh
Artemisia annua L. (Asteraceae ...
Artemisia annua
FEBS Lett.
580
1411-1416
2006
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