BRENDA - Enzyme Database show
show all sequences of 1.14.14.115

Triterpene functional genomics in Licorice for identification of CYP72A154 involved in the biosynthesis of glycyrrhizin

Seki, H.; Sawai, S.; Ohyama, K.; Mizutani, M.; Ohnishi, T.; Sudo, H.; Fukushima, E.O.; Akashi, T.; Aoki, T.; Saito, K.; Muranaka, T.; Plant Cell 23, 4112-4123 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli DH10Bac cells and in Saccharomyces cerevisiae strain BJ2168; expressed in Saccharomyces cerevisiae strain BJ2168 that endogenously produces 11-oxo-beta-amyrin
Glycyrrhiza uralensis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
Glycyrrhiza uralensis
the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products
30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycyrrhiza uralensis
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
colon
-
Glycyrrhiza uralensis
-
additional information
not detected in leaves
Glycyrrhiza uralensis
-
root
-
Glycyrrhiza uralensis
-
stem
-
Glycyrrhiza uralensis
-
stolon
-
Glycyrrhiza uralensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
single oxidation reaction catalyzed by CYP72A154
716523
Glycyrrhiza uralensis
30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products
716523
Glycyrrhiza uralensis
30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
-
716523
Glycyrrhiza uralensis
glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
single oxidation reaction catalyzed by CYP72A154
716523
Glycyrrhiza uralensis
glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
glycyrrhetaldehyde + O2 + [reduced NADPH-hemoprotein reductase]
-
716523
Glycyrrhiza uralensis
glycyrrhetinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli DH10Bac cells and in Saccharomyces cerevisiae strain BJ2168; expressed in Saccharomyces cerevisiae strain BJ2168 that endogenously produces 11-oxo-beta-amyrin
Glycyrrhiza uralensis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
Glycyrrhiza uralensis
the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products
30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
colon
-
Glycyrrhiza uralensis
-
additional information
not detected in leaves
Glycyrrhiza uralensis
-
root
-
Glycyrrhiza uralensis
-
stem
-
Glycyrrhiza uralensis
-
stolon
-
Glycyrrhiza uralensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
single oxidation reaction catalyzed by CYP72A154
716523
Glycyrrhiza uralensis
30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
the enzyme mainly catalyzes the C-30 hydroxylation of 11-oxo-beta-amyrin to yield 30-hydroxy-11-oxo-beta-amyrin as the major product, accompanied by its isomers as minor products
716523
Glycyrrhiza uralensis
30-hydroxy-11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
-
716523
Glycyrrhiza uralensis
glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
30-hydroxy-11-oxo-beta-amyrin + O2 + [reduced NADPH-hemoprotein reductase]
single oxidation reaction catalyzed by CYP72A154
716523
Glycyrrhiza uralensis
glycyrrhetaldehyde + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
glycyrrhetaldehyde + O2 + [reduced NADPH-hemoprotein reductase]
-
716523
Glycyrrhiza uralensis
glycyrrhetinate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
the beta-amyrin 11-oxidase gene CYP88D6 is the initial P450 gene in glycyrrhizin biosynthesis. CYP72A154 is responsible for C-30 oxidation of beta-amyrin in the glycyrrhizin pathway; the enzyme is involved in the biosynthesis of glycyrrhizin
Glycyrrhiza uralensis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the beta-amyrin 11-oxidase gene CYP88D6 is the initial P450 gene in glycyrrhizin biosynthesis. CYP72A154 is responsible for C-30 oxidation of beta-amyrin in the glycyrrhizin pathway; the enzyme is involved in the biosynthesis of glycyrrhizin
Glycyrrhiza uralensis
Other publictions for EC 1.14.14.115
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
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1
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4
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4
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4
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5
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1
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2
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1
2
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4
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5
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1
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1
1
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716523
Seki
Triterpene functional genomics ...
Glycyrrhiza uralensis
Plant Cell
23
4112-4123
2011
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1
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1
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4
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5
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5
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1
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1
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5
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5
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1
1
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