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Literature summary for 1.14.14.115 extracted from

  • Tamura, K.; Teranishi, Y.; Ueda, S.; Suzuki, H.; Kawano, N.; Yoshimatsu, K.; Saito, K.; Kawahara, N.; Muranaka, T.; Seki, H.
    Cytochrome P450 monooxygenase CYP716A141 is a unique beta-amyrin C-16beta oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus (2017), Plant Cell Physiol., 58, 874-884 .
    View publication on PubMed
Search for more literature for 1.14.14.115

Cloned(Commentary)

Cloned (Comment) Organism
gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels Platycodon grandiflorus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 Platycodon grandiflorus hydroxylation at C-16beta, main activity of enzyme CYP716A141 maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 Platycodon grandiflorus hydroxylation at C-28, reaction of EC 1.14.13.201 oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2 Platycodon grandiflorus hydroxylation at C-28 cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 Platycodon grandiflorus hydroxylation at C-16beta cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Platycodon grandiflorus A0A1I9Q5Z0
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Platycodon grandiflorus
-
additional information transcriptome analysis in different tissues using RNA sequencing technology, overview Platycodon grandiflorus
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petal
-
 Platycodon grandiflorus
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root the enzyme is highly expressed in roots Platycodon grandiflorus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-16beta, main activity of enzyme CYP716A141 Platycodon grandiflorus maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-28, reaction of EC 1.14.13.201 Platycodon grandiflorus oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-28 Platycodon grandiflorus cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
additional information CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry Platycodon grandiflorus ?
-
 ?
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation at C-16beta Platycodon grandiflorus cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

Synonyms Comment Organism
beta-amyrin C-16beta oxidase
-
 Platycodon grandiflorus
C-16beta oxidase
-
 Platycodon grandiflorus
CYP716A141
-
 Platycodon grandiflorus
More cf. EC 1.14.13.201, beta-amyrin C-28 oxidase Platycodon grandiflorus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 in vivo assay at Platycodon grandiflorus

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
-
 Platycodon grandiflorus
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Platycodon grandiflorus

General Information

General Information Comment Organism
evolution the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases Platycodon grandiflorus