BRENDA - Enzyme Database show
show all sequences of 1.14.14.115

Cytochrome P450 monooxygenase CYP716A141 is a unique beta-amyrin C-16beta oxidase involved in triterpenoid saponin biosynthesis in Platycodon grandiflorus

Tamura, K.; Teranishi, Y.; Ueda, S.; Suzuki, H.; Kawano, N.; Yoshimatsu, K.; Saito, K.; Kawahara, N.; Muranaka, T.; Seki, H.; Plant Cell Physiol. 58, 874-884 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels
Platycodon grandiflorus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-16beta, main activity of enzyme CYP716A141
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-28, reaction of EC 1.14.13.201
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-28
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-16beta
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Platycodon grandiflorus
A0A1I9Q5Z0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Platycodon grandiflorus
-
additional information
transcriptome analysis in different tissues using RNA sequencing technology, overview
Platycodon grandiflorus
-
petal
-
Platycodon grandiflorus
-
root
the enzyme is highly expressed in roots
Platycodon grandiflorus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta, main activity of enzyme CYP716A141
746048
Platycodon grandiflorus
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28, reaction of EC 1.14.13.201
746048
Platycodon grandiflorus
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry
746048
Platycodon grandiflorus
?
-
-
-
-
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
in vivo assay at
Platycodon grandiflorus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Platycodon grandiflorus
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Platycodon grandiflorus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP716A141, DNA and amino acid sequence determination and analysis, phylogenetic tree, quantitative real-time PCR enzyme expression analysis, recombinant coexpression of genes CYP716A140v2 and CYP716A141 in Saccharomyces cerevisiae strain 10, that shows increased cochalic acid levels
Platycodon grandiflorus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Platycodon grandiflorus
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Platycodon grandiflorus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-16beta, main activity of enzyme CYP716A141
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-28, reaction of EC 1.14.13.201
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-28
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
Platycodon grandiflorus
hydroxylation at C-16beta
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Platycodon grandiflorus
-
additional information
transcriptome analysis in different tissues using RNA sequencing technology, overview
Platycodon grandiflorus
-
petal
-
Platycodon grandiflorus
-
root
the enzyme is highly expressed in roots
Platycodon grandiflorus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta, main activity of enzyme CYP716A141
746048
Platycodon grandiflorus
maniladiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28, reaction of EC 1.14.13.201
746048
Platycodon grandiflorus
oleanolic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
maniladiol + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-28
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
CYP716A141 is capable of catalyzing oxidation at both C-16beta and C-28, metabolites analysis by mass spectrometry
746048
Platycodon grandiflorus
?
-
-
-
-
oleanolic acid + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation at C-16beta
746048
Platycodon grandiflorus
cochalic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
in vivo assay at
Platycodon grandiflorus
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases
Platycodon grandiflorus
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the CYP716A subfamily of cytochrome P450 monooxygenases
Platycodon grandiflorus
Other publictions for EC 1.14.14.115
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
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716523
Seki
Triterpene functional genomics ...
Glycyrrhiza uralensis
Plant Cell
23
4112-4123
2011
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1
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1
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1
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5
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5
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1
1
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