BRENDA - Enzyme Database show
show all sequences of 1.14.14.116

avnA, a gene encoding a cytochrome P-450 monooxygenase, is involved in the conversion of averantin to averufin in aflatoxin biosynthesis in Aspergillus parasiticus

Yu, J.; Chang, P.K.; Cary, J.W.; Bhatnagar, D.; Cleveland, T.E.; Appl. Environ. Microbiol. 63, 1349-1356 (1997)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56300
-
x * 56300, calculated from amino acid sequence; x * 56300, calculated from sequence
Aspergillus parasiticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SU1-N3
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SU1-N3
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
-
5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SRRC 143
-
5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus parasiticus
-
ATCC 56775
-
Aspergillus parasiticus
Q12732
-
-
Aspergillus parasiticus SRRC 143
-
ATCC 56775
-
Aspergillus parasiticus SU1-N3
Q12732
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus SU1-N3
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus SU1-N3
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus SU1-N3
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus SU1-N3
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
averantin + [reduced NADPH-hemoprotein reductase] + O2
-
721321
Aspergillus parasiticus
5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
averantin + [reduced NADPH-hemoprotein reductase] + O2
-
721321
Aspergillus parasiticus SRRC 143
5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 56300, calculated from amino acid sequence; x * 56300, calculated from sequence
Aspergillus parasiticus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
a cytochrome P-450-type monooxygenase
Aspergillus parasiticus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
a cytochrome P-450-type monooxygenase
Aspergillus parasiticus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
56300
-
x * 56300, calculated from sequence
Aspergillus parasiticus
56300
-
x * 56300, calculated from amino acid sequence
Aspergillus parasiticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SU1-N3
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SU1-N3
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus
-
5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
averantin + [reduced NADPH-hemoprotein reductase] + O2
Aspergillus parasiticus SRRC 143
-
5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus SU1-N3
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus SU1-N3
(1'S,5'R)-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme catalyzes a reaction in the aflatoxin biosynthesis pathway. It specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus SU1-N3
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(1'S)-averantin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme specifically hydroxylates (1'S)-averantin to the diastereomers (1'S,5'R)-hydroxyaverantin and (1'S,5'S)-hydroxyaverantin
721321
Aspergillus parasiticus SU1-N3
(1'S,5'S)-5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
averantin + [reduced NADPH-hemoprotein reductase] + O2
-
721321
Aspergillus parasiticus
5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
averantin + [reduced NADPH-hemoprotein reductase] + O2
-
721321
Aspergillus parasiticus SRRC 143
5'-hydroxyaverantin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 56300, calculated from sequence
Aspergillus parasiticus
?
x * 56300, calculated from amino acid sequence
Aspergillus parasiticus
General Information
General Information
Commentary
Organism
malfunction
disruption of the avnA gene in the wild type aflatoxigenic Aspergillus parasiticus strain SU1-N3 results in a nonaflatoxigenic mutant which accumulates a bright yellow pigment. Norsolorinic acid and averantin are not converted to aflatoxin by the mutant whereas 5'-hydroxyaverantin, averufanin, averufin, versicolorin A, sterigmatocystin, and O-methylsterigmatocystin are converted to aflatoxins
Aspergillus parasiticus
metabolism
the enzyme is involved in the conversion of averantin to averufin in the aflatoxin biosynthetic pathway
Aspergillus parasiticus
General Information (protein specific)
General Information
Commentary
Organism
malfunction
disruption of the avnA gene in the wild type aflatoxigenic Aspergillus parasiticus strain SU1-N3 results in a nonaflatoxigenic mutant which accumulates a bright yellow pigment. Norsolorinic acid and averantin are not converted to aflatoxin by the mutant whereas 5'-hydroxyaverantin, averufanin, averufin, versicolorin A, sterigmatocystin, and O-methylsterigmatocystin are converted to aflatoxins
Aspergillus parasiticus
metabolism
the enzyme is involved in the conversion of averantin to averufin in the aflatoxin biosynthetic pathway
Aspergillus parasiticus
Other publictions for EC 1.14.14.116
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
721364
Yabe
Enzyme reactions and genes in ...
Aspergillus parasiticus
Appl. Microbiol. Biotechnol.
64
745-755
2004
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1
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2
-
1
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4
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1
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2
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4
-
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-
721321
Yu
avnA, a gene encoding a cytoch ...
Aspergillus parasiticus, Aspergillus parasiticus SRRC 143, Aspergillus parasiticus SU1-N3
Appl. Environ. Microbiol.
63
1349-1356
1997
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1
6
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6
-
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-
-
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10
1
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-
1
-
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-
1
-
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2
6
-
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10
2
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2
2
-
-
-
721316
Yabe
Stereochemistry during aflatox ...
Aspergillus parasiticus, Aspergillus parasiticus NIAH-26
Appl. Environ. Microbiol.
59
2486-2492
1993
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2
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5
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9
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9
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-
-
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2
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5
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9
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