Cloned (Comment) | Organism |
---|---|
gene CYP716A53v2, recombinant overexpression in Panx ginseng roots using the Agrobacterium tumefaciens strain GV3101 transformation method, expression under control of CaMV 35S promoter, subcloning in Escherichia coli strain DH5alpha | Panax ginseng |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of transgenic Panax ginseng lines overexpressing CYP716A53v2 gene or silencing CYP716A53v2 gene via RNA interference (RNAi), analyis changes in ginsenoside profiles, overview. Production of protopanaxadiol- and protopanaxatriol-group ginsenosides can be altered by changing the expression of CYP716A53v2 in transgenic Panax ginseng. Changing the expression of CYP716A53v2 affects the downstream genes (glycosyltransferases) | Panax ginseng |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2 | Panax ginseng | - |
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Panax ginseng | I7CT85 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Panax ginseng | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2 | - |
Panax ginseng | protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP716A53v2 | - |
Panax ginseng |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Panax ginseng | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Panax ginseng |
General Information | Comment | Organism |
---|---|---|
metabolism | two cytochrome P450 (CYP) genes, CYP716A47 and CYP716A53v2, are critical for the production of protopanaxadiol and protopanaxatriol aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes protopanaxatriol production from protopanaxadiol in Panax ginseng, biosynthetic pathway for ginsenosides in Panax ginseng, overview | Panax ginseng |