BRENDA - Enzyme Database show
show all sequences of 1.14.14.121

Alteration of Panax ginseng saponin composition by overexpression and RNA interference of the protopanaxadiol 6-hydroxylase gene (CYP716A53v2)

Park, S.B.; Chun, J.H.; Ban, Y.W.; Han, J.Y.; Choi, Y.E.; J. Ginseng Res. 40, 47-54 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP716A53v2, recombinant overexpression in Panx ginseng roots using the Agrobacterium tumefaciens strain GV3101 transformation method, expression under control of CaMV 35S promoter, subcloning in Escherichia coli strain DH5alpha
Panax ginseng
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of transgenic Panax ginseng lines overexpressing CYP716A53v2 gene or silencing CYP716A53v2 gene via RNA interference (RNAi), analyis changes in ginsenoside profiles, overview. Production of protopanaxadiol- and protopanaxatriol-group ginsenosides can be altered by changing the expression of CYP716A53v2 in transgenic Panax ginseng. Changing the expression of CYP716A53v2 affects the downstream genes (glycosyltransferases)
Panax ginseng
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
-
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Panax ginseng
I7CT85
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Panax ginseng
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
-
745480
Panax ginseng
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Panax ginseng
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Panax ginseng
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP716A53v2, recombinant overexpression in Panx ginseng roots using the Agrobacterium tumefaciens strain GV3101 transformation method, expression under control of CaMV 35S promoter, subcloning in Escherichia coli strain DH5alpha
Panax ginseng
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Panax ginseng
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Panax ginseng
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of transgenic Panax ginseng lines overexpressing CYP716A53v2 gene or silencing CYP716A53v2 gene via RNA interference (RNAi), analyis changes in ginsenoside profiles, overview. Production of protopanaxadiol- and protopanaxatriol-group ginsenosides can be altered by changing the expression of CYP716A53v2 in transgenic Panax ginseng. Changing the expression of CYP716A53v2 affects the downstream genes (glycosyltransferases)
Panax ginseng
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
-
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Panax ginseng
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
-
745480
Panax ginseng
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
two cytochrome P450 (CYP) genes, CYP716A47 and CYP716A53v2, are critical for the production of protopanaxadiol and protopanaxatriol aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes protopanaxatriol production from protopanaxadiol in Panax ginseng, biosynthetic pathway for ginsenosides in Panax ginseng, overview
Panax ginseng
General Information (protein specific)
General Information
Commentary
Organism
metabolism
two cytochrome P450 (CYP) genes, CYP716A47 and CYP716A53v2, are critical for the production of protopanaxadiol and protopanaxatriol aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes protopanaxatriol production from protopanaxadiol in Panax ginseng, biosynthetic pathway for ginsenosides in Panax ginseng, overview
Panax ginseng
Other publictions for EC 1.14.14.121
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745480
Park
Alteration of Panax ginseng s ...
Panax ginseng
J. Ginseng Res.
40
47-54
2016
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1
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1
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2
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1
2
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1
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1
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1
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1
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1
1
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726485
Dai
Producing aglycons of ginsenos ...
Panax ginseng
Sci. Rep.
4
3698
2014
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1
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1
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5
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1
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1
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1
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1
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745012
Wang
Identification of the protopa ...
Panax quinquefolius
Funct. Integr. Genomics
14
559-570
2014
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1
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1
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1
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5
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1
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2
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1
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1
2
2
1
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726145
Han
Cytochrome P450 CYP716A53v2 ca ...
Panax ginseng
Plant Cell Physiol.
53
1535-1545
2012
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1
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1
1
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4
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6
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1
1
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1
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1
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6
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1
1
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1
1
1
1
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724615
Yue
Protopanaxadiol 6-hydroxylase ...
Panax notoginseng
Biotechnol. Bioeng.
100
933-940
2008
1
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5
1
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2
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5
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2
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1
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1
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5
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