BRENDA - Enzyme Database show
show all sequences of 1.14.14.126

The involvement of beta-amyrin 28-oxidase (Cyp716a52v2) in oleanane-type ginsenoside biosynthesis in Panax ginseng

Han, J.; Kim, M.; Ban, Y.; Hwang, H.; Choi, Y.; Plant Cell Physiol. 54, 2034-2046 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
coexpression of PNY1 and CYP716A52v2 in yeast. Transgenic Panax ginseng plants constitutively overexpressing CYP716A52v2 and expressing a CYP716A52v2-RNAi are generated using Agrobacterium tumefaciens mediated genetic transformation; expression in Saccharomyces cerevisiae
Panax ginseng
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
x * 54000, calculated; x * 54000, calculated from sequence
Panax ginseng
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Panax ginseng
I7C6E8
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
very low levels of mRNA in other organs than rhizome
Panax ginseng
-
rhizome
-
Panax ginseng
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
732623
Panax ginseng
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro
732623
Panax ginseng
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 54000, calculated; x * 54000, calculated from sequence
Panax ginseng
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Panax ginseng
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Panax ginseng
Cloned(Commentary) (protein specific)
Commentary
Organism
coexpression of PNY1 and CYP716A52v2 in yeast. Transgenic Panax ginseng plants constitutively overexpressing CYP716A52v2 and expressing a CYP716A52v2-RNAi are generated using Agrobacterium tumefaciens mediated genetic transformation; expression in Saccharomyces cerevisiae
Panax ginseng
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
54000
-
x * 54000, calculated; x * 54000, calculated from sequence
Panax ginseng
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
Panax ginseng
the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
very low levels of mRNA in other organs than rhizome
Panax ginseng
-
rhizome
-
Panax ginseng
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
732623
Panax ginseng
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro
732623
Panax ginseng
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 54000, calculated; x * 54000, calculated from sequence
Panax ginseng
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Panax ginseng
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Panax ginseng
Expression
Organism
Commentary
Expression
Panax ginseng
CYP716A52v2 mRNA does not respond to methyl jasmonate treatment during the entire culture period
additional information
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro
Panax ginseng
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the biosynthesis of oleanane-type triterpenoids, such as ginsenoside Ro
Panax ginseng
Expression (protein specific)
Organism
Commentary
Expression
Panax ginseng
CYP716A52v2 mRNA does not respond to methyl jasmonate treatment during the entire culture period
additional information
Other publictions for EC 1.14.14.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744248
Zhan
Cytochrome P450 CYP716A254 ca ...
Anemone flaccida
Biochem. Biophys. Res. Commun.
495
1271-1277
2018
-
-
1
-
-
-
-
-
2
-
-
1
-
3
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
1
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
745002
Yasumoto
Functional characterization o ...
Solanum lycopersicum
Front. Plant Sci.
8
21
2017
-
-
1
-
-
-
-
-
-
-
-
6
-
2
-
-
-
-
-
4
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
6
-
-
6
-
-
-
-
-
-
-
-
-
-
2
4
-
-
-
745906
Misra
Two CYP716A subfamily cytochr ...
Ocimum basilicum
New Phytol.
214
706-720
2017
-
-
1
-
1
-
-
-
-
-
-
4
-
4
-
-
-
-
-
3
-
-
5
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
3
-
-
5
-
-
-
-
-
-
-
-
-
1
3
3
1
-
-
746004
Jo
beta-Amyrin synthase (EsBAS) ...
Eleutherococcus senticosus
Phytochemistry
135
53-63
2017
-
-
1
-
-
-
-
-
1
1
-
4
-
7
-
-
-
-
-
2
-
-
7
1
-
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
2
-
-
7
1
-
-
-
-
-
-
-
-
1
2
2
1
-
-
746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
5
-
-
-
-
-
4
-
-
11
-
1
-
-
-
-
-
-
2
-
-
-
-
-
2
4
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
8
-
-
11
-
2
-
-
-
-
-
-
-
-
2
3
-
-
-
746049
Han
Transcriptomic analysis of Ka ...
Kalopanax septemlobus
Plant Cell Physiol.
59
319-330
2017
-
-
1
-
-
-
-
-
2
1
-
1
-
7
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
3
-
-
-
-
-
1
3
-
-
-
-
-
-
-
2
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
2
-
-
-
746060
Tamura
CYP716A179 functions as a tri ...
Glycyrrhiza uralensis, Glycyrrhiza uralensis 308-19
Plant Cell Rep.
36
437-445
2017
-
-
1
-
-
-
-
-
-
-
-
16
-
6
-
-
-
-
-
6
-
-
18
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
16
-
-
-
-
-
6
-
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
748772
Andre
Multifunctional oxidosqualene ...
Malus domestica
New Phytol.
211
1279-1294
2016
-
-
1
-
-
-
-
-
-
-
-
5
-
2
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
732623
Han
The involvement of beta-amyrin ...
Panax ginseng
Plant Cell Physiol.
54
2034-2046
2013
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
-
-
-
-
2
-
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
2
1
1
-
-
-
1
-
-
-
1
1
1
1
-
-
732622
Fukushima
CYP716A subfamily members are ...
Medicago truncatula, Vitis vinifera
Plant Cell Physiol.
52
2050-2061
2011
-
-
2
-
-
-
-
-
-
-
-
7
-
6
-
-
-
4
-
2
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
4
-
-
19
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
732627
Carelli
Medicago truncatula CYP716A12 ...
Medicago truncatula
Plant Cell
23
3070-3081
2011
-
-
1
-
-
-
-
-
1
-
1
-
-
5
-
-
-
-
-
5
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
5
-
-
2
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-