BRENDA - Enzyme Database show
show all sequences of 1.14.14.126

Medicago truncatula CYP716A12 is a multifunctional oxidase involved in the biosynthesis of hemolytic saponins

Carelli, M.; Biazzi, E.; Panara, F.; Tava, A.; Scaramelli, L.; Porceddu, A.; Graham, N.; Odoardi, M.; Piano, E.; Arcioni, S.; May, S.; Scotti, C.; Calderini, O.; Plant Cell 23, 3070-3081 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Medicago truncatula
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Medicago truncatula
-
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
x * 57000, SDS-PAGE
Medicago truncatula
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Medicago truncatula
F8J4R7
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
highest expression of the gene
Medicago truncatula
-
leaf
significant increase in expression is observed in the reproductive stages, with a maximum expression at flowering
Medicago truncatula
-
additional information
expression of the gene occurs in all the organs and the developmental stages
Medicago truncatula
-
root
-
Medicago truncatula
-
stem
-
Medicago truncatula
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
732627
Medicago truncatula
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
CYP716A12 mainly catalyzes the sequential three-step oxidation at the C-28 position necessary to transform beta-amyrin into oleanolic acid
-
-
?
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2
-
732627
Medicago truncatula
oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 57000, SDS-PAGE
Medicago truncatula
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Medicago truncatula
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Medicago truncatula
-
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
57000
-
x * 57000, SDS-PAGE
Medicago truncatula
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
highest expression of the gene
Medicago truncatula
-
leaf
significant increase in expression is observed in the reproductive stages, with a maximum expression at flowering
Medicago truncatula
-
additional information
expression of the gene occurs in all the organs and the developmental stages
Medicago truncatula
-
root
-
Medicago truncatula
-
stem
-
Medicago truncatula
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
overall reaction
732627
Medicago truncatula
oleanolate + [oxidized NADPH-hemoprotein reductase] + H2O
CYP716A12 mainly catalyzes the sequential three-step oxidation at the C-28 position necessary to transform beta-amyrin into oleanolic acid
-
-
?
erythrodiol + [reduced NADPH-hemoprotein reductase] + O2
-
732627
Medicago truncatula
oleanolic aldehyde + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 57000, SDS-PAGE
Medicago truncatula
General Information
General Information
Commentary
Organism
physiological function
enzyme is responsible for an early step in the saponin biosynthetic pathway. Mutants in CYP716A12 are unable to produce hemolytic saponins and only synthetize soyasaponins. CYP716A12 catalyzes the oxidation of beta-amyrin and erythrodiol at the C-28 position, yielding oleanolic acid
Medicago truncatula
General Information (protein specific)
General Information
Commentary
Organism
physiological function
enzyme is responsible for an early step in the saponin biosynthetic pathway. Mutants in CYP716A12 are unable to produce hemolytic saponins and only synthetize soyasaponins. CYP716A12 catalyzes the oxidation of beta-amyrin and erythrodiol at the C-28 position, yielding oleanolic acid
Medicago truncatula
Other publictions for EC 1.14.14.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744248
Zhan
Cytochrome P450 CYP716A254 ca ...
Anemone flaccida
Biochem. Biophys. Res. Commun.
495
1271-1277
2018
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1
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2
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1
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3
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1
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2
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1
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1
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2
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1
2
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2
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1
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1
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2
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1
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1
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2
2
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-
745002
Yasumoto
Functional characterization o ...
Solanum lycopersicum
Front. Plant Sci.
8
21
2017
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-
1
-
-
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-
-
-
-
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6
-
2
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4
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6
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2
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6
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6
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6
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2
4
-
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-
745906
Misra
Two CYP716A subfamily cytochr ...
Ocimum basilicum
New Phytol.
214
706-720
2017
-
-
1
-
1
-
-
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4
-
4
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3
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5
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3
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1
3
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1
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4
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3
-
-
5
-
-
-
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-
-
-
-
1
3
3
1
-
-
746004
Jo
beta-Amyrin synthase (EsBAS) ...
Eleutherococcus senticosus
Phytochemistry
135
53-63
2017
-
-
1
-
-
-
-
-
1
1
-
4
-
7
-
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2
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7
1
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3
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1
3
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1
1
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4
-
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-
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2
-
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7
1
-
-
-
-
-
-
-
-
1
2
2
1
-
-
746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
5
-
-
-
-
-
4
-
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11
-
1
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2
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2
4
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4
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8
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11
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2
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2
3
-
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-
746049
Han
Transcriptomic analysis of Ka ...
Kalopanax septemlobus
Plant Cell Physiol.
59
319-330
2017
-
-
1
-
-
-
-
-
2
1
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1
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7
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1
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1
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3
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1
3
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2
1
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1
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1
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1
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2
2
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746060
Tamura
CYP716A179 functions as a tri ...
Glycyrrhiza uralensis, Glycyrrhiza uralensis 308-19
Plant Cell Rep.
36
437-445
2017
-
-
1
-
-
-
-
-
-
-
-
16
-
6
-
-
-
-
-
6
-
-
18
-
-
-
-
-
-
-
-
2
-
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1
2
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16
-
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6
-
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18
-
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-
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-
-
-
-
-
-
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-
-
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-
748772
Andre
Multifunctional oxidosqualene ...
Malus domestica
New Phytol.
211
1279-1294
2016
-
-
1
-
-
-
-
-
-
-
-
5
-
2
-
-
-
-
-
1
-
-
6
-
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-
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-
1
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1
1
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5
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1
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6
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1
1
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-
732623
Han
The involvement of beta-amyrin ...
Panax ginseng
Plant Cell Physiol.
54
2034-2046
2013
-
-
1
-
-
-
-
-
-
-
1
1
-
5
-
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2
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2
1
1
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1
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1
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1
1
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2
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2
1
1
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-
1
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-
1
1
1
1
-
-
732622
Fukushima
CYP716A subfamily members are ...
Medicago truncatula, Vitis vinifera
Plant Cell Physiol.
52
2050-2061
2011
-
-
2
-
-
-
-
-
-
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-
7
-
6
-
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4
-
2
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19
-
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3
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7
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4
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19
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732627
Carelli
Medicago truncatula CYP716A12 ...
Medicago truncatula
Plant Cell
23
3070-3081
2011
-
-
1
-
-
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-
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1
-
1
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5
-
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5
-
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2
1
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1
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1
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1
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5
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2
1
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1
1
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