BRENDA - Enzyme Database show
show all sequences of 1.14.14.126

Cytochrome P450 CYP716A254 catalyzes the formation of oleanolic acid from beta-amyrin during oleanane-type triterpenoid saponins biosynthesis in Anemone flaccida

Zhan, C.; Ahmed, S.; Hu, S.; Dong, S.; Cai, Q.; Yang, T.; Wang, X.; Li, X.; Hu, X.; Biochem. Biophys. Res. Commun. 495, 1271-1277 (2018)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP716A254, DNA and amino acid sequence determination and analysis, phylogenetic tree, heterologous expression in Saccharomyces cerevisiae strain WAT11 results in oxidation of beta-amyrin at the C-28 position to oleanolic acid production
Anemone flaccida
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Anemone flaccida
16020
-
microsome
-
Anemone flaccida
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Anemone flaccida
overall reaction
oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Anemone flaccida
I7C6E8
collected from Tonggubao Village, Machi Town, Changyang County, Hubei Province of China in May of 2014
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
rhizome
-
Anemone flaccida
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
overall reaction
744248
Anemone flaccida
oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
additional information
metabolite identification by mass spectrometry
744248
Anemone flaccida
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Anemone flaccida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Anemone flaccida
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Anemone flaccida
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Anemone flaccida
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP716A254, DNA and amino acid sequence determination and analysis, phylogenetic tree, heterologous expression in Saccharomyces cerevisiae strain WAT11 results in oxidation of beta-amyrin at the C-28 position to oleanolic acid production
Anemone flaccida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Anemone flaccida
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Anemone flaccida
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Anemone flaccida
16020
-
microsome
-
Anemone flaccida
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Anemone flaccida
overall reaction
oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
rhizome
-
Anemone flaccida
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
overall reaction
744248
Anemone flaccida
oleanolate + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
additional information
metabolite identification by mass spectrometry
744248
Anemone flaccida
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Anemone flaccida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Anemone flaccida
General Information
General Information
Commentary
Organism
metabolism
cytochrome P450 monooxygenase CYP716A254 is involved in oleanane-type triterpenoid saponins biosynthesis, i.e. the biosynthesis of oleanolic acid, which involves cyclization of 2,3-oxidosqualene to the oleanane-type triterpenoid skeleton, followed by a series of oxidation reactions catalyzed by cytochrome P450 monooxygenase (CYP450), Proposed biosynthetic pathway for oleanolic acid in Anemone flaccida, overview
Anemone flaccida
physiological function
cytochrome P450 monooxygenase CYP716A254 catalyzes the formation of oleanolic acid from beta-amyrin during oleanane-type triterpenoid saponins biosynthesis. Oleanolic acid is natural triterpenoid in plants with diverse biological activities
Anemone flaccida
General Information (protein specific)
General Information
Commentary
Organism
metabolism
cytochrome P450 monooxygenase CYP716A254 is involved in oleanane-type triterpenoid saponins biosynthesis, i.e. the biosynthesis of oleanolic acid, which involves cyclization of 2,3-oxidosqualene to the oleanane-type triterpenoid skeleton, followed by a series of oxidation reactions catalyzed by cytochrome P450 monooxygenase (CYP450), Proposed biosynthetic pathway for oleanolic acid in Anemone flaccida, overview
Anemone flaccida
physiological function
cytochrome P450 monooxygenase CYP716A254 catalyzes the formation of oleanolic acid from beta-amyrin during oleanane-type triterpenoid saponins biosynthesis. Oleanolic acid is natural triterpenoid in plants with diverse biological activities
Anemone flaccida
Other publictions for EC 1.14.14.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744248
Zhan
Cytochrome P450 CYP716A254 ca ...
Anemone flaccida
Biochem. Biophys. Res. Commun.
495
1271-1277
2018
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2
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745002
Yasumoto
Functional characterization o ...
Solanum lycopersicum
Front. Plant Sci.
8
21
2017
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6
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6
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6
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2
4
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745906
Misra
Two CYP716A subfamily cytochr ...
Ocimum basilicum
New Phytol.
214
706-720
2017
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1
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3
3
1
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746004
Jo
beta-Amyrin synthase (EsBAS) ...
Eleutherococcus senticosus
Phytochemistry
135
53-63
2017
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1
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7
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1
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3
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7
1
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1
2
2
1
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746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
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1
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4
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5
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4
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11
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1
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2
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2
4
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4
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8
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11
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2
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2
3
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746049
Han
Transcriptomic analysis of Ka ...
Kalopanax septemlobus
Plant Cell Physiol.
59
319-330
2017
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2
1
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7
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3
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3
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1
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2
2
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746060
Tamura
CYP716A179 functions as a tri ...
Glycyrrhiza uralensis, Glycyrrhiza uralensis 308-19
Plant Cell Rep.
36
437-445
2017
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1
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-
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16
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6
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6
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18
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2
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16
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6
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18
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748772
Andre
Multifunctional oxidosqualene ...
Malus domestica
New Phytol.
211
1279-1294
2016
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1
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6
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1
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732623
Han
The involvement of beta-amyrin ...
Panax ginseng
Plant Cell Physiol.
54
2034-2046
2013
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1
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1
1
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5
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2
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1
1
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1
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1
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1
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2
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2
1
1
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1
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1
1
1
1
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732622
Fukushima
CYP716A subfamily members are ...
Medicago truncatula, Vitis vinifera
Plant Cell Physiol.
52
2050-2061
2011
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2
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7
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19
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19
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732627
Carelli
Medicago truncatula CYP716A12 ...
Medicago truncatula
Plant Cell
23
3070-3081
2011
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