Cloned (Comment) | Organism |
---|---|
gene CYP716A94, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain WAT21 microsomes, coexpression with Arabidopsis thaliana NADPH-CYP reductase leads to production of oleanolic acid, mass spectrometric analysis | Kalopanax septemlobus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | a transmembrane enzyme | Kalopanax septemlobus | 16020 | - |
microsome | - |
Kalopanax septemlobus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | bound in heme | Kalopanax septemlobus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | Kalopanax septemlobus | - |
oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kalopanax septemlobus | A0A0S2II38 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 | - |
Kalopanax septemlobus | oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-amyrin 28-oxidase | - |
Kalopanax septemlobus |
CYP716A94 | - |
Kalopanax septemlobus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vivo assay at | Kalopanax septemlobus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Kalopanax septemlobus | |
heme | - |
Kalopanax septemlobus | |
NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Kalopanax septemlobus |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes a step in the putative saponin biosynthetic pathway from 2,3-oxidosqualene to hederagenin saponin, overview. Two enzymes (beta-amyrin synthase and beta-amyrin 28-oxidase) are essential for oleanane-type saponin biosynthesis from 2,3-oxidosqualene | Kalopanax septemlobus |
physiological function | CYP716A94 is a beta-amyrin 28-oxidase for the production of oleanolic acid | Kalopanax septemlobus |