BRENDA - Enzyme Database show
show all sequences of 1.14.14.126

Transcriptomic analysis of Kalopanax septemlobus and characterization of KsBAS, CYP716A94, and CYP72A397 genes involved in hederagenin saponin biosynthesis

Han, J.Y.; Chun, J.H.; O, S.A.; Park, S.B.; Hwang, H.S.; Lee, H.; Choi, Y.E.; Plant Cell Physiol. 59, 319-330 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene CYP716A94, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain WAT21 microsomes, coexpression with Arabidopsis thaliana NADPH-CYP reductase leads to production of oleanolic acid, mass spectrometric analysis
Kalopanax septemlobus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
a transmembrane enzyme
Kalopanax septemlobus
16020
-
microsome
-
Kalopanax septemlobus
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
bound in heme
Kalopanax septemlobus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Kalopanax septemlobus
-
oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Kalopanax septemlobus
A0A0S2II38
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746049
Kalopanax septemlobus
oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
in vivo assay at
Kalopanax septemlobus
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Kalopanax septemlobus
heme
-
Kalopanax septemlobus
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Kalopanax septemlobus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene CYP716A94, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Saccharomyces cerevisiae strain WAT21 microsomes, coexpression with Arabidopsis thaliana NADPH-CYP reductase leads to production of oleanolic acid, mass spectrometric analysis
Kalopanax septemlobus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Kalopanax septemlobus
heme
-
Kalopanax septemlobus
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Kalopanax septemlobus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
a transmembrane enzyme
Kalopanax septemlobus
16020
-
microsome
-
Kalopanax septemlobus
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
bound in heme
Kalopanax septemlobus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
Kalopanax septemlobus
-
oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-amyrin + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
746049
Kalopanax septemlobus
oleanolate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
in vivo assay at
Kalopanax septemlobus
General Information
General Information
Commentary
Organism
metabolism
the enzyme catalyzes a step in the putative saponin biosynthetic pathway from 2,3-oxidosqualene to hederagenin saponin, overview. Two enzymes (beta-amyrin synthase and beta-amyrin 28-oxidase) are essential for oleanane-type saponin biosynthesis from 2,3-oxidosqualene
Kalopanax septemlobus
physiological function
CYP716A94 is a beta-amyrin 28-oxidase for the production of oleanolic acid
Kalopanax septemlobus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme catalyzes a step in the putative saponin biosynthetic pathway from 2,3-oxidosqualene to hederagenin saponin, overview. Two enzymes (beta-amyrin synthase and beta-amyrin 28-oxidase) are essential for oleanane-type saponin biosynthesis from 2,3-oxidosqualene
Kalopanax septemlobus
physiological function
CYP716A94 is a beta-amyrin 28-oxidase for the production of oleanolic acid
Kalopanax septemlobus
Other publictions for EC 1.14.14.126
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
744248
Zhan
Cytochrome P450 CYP716A254 ca ...
Anemone flaccida
Biochem. Biophys. Res. Commun.
495
1271-1277
2018
-
-
1
-
-
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2
-
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1
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3
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1
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2
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1
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1
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2
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1
2
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2
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1
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1
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2
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1
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1
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2
2
-
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-
745002
Yasumoto
Functional characterization o ...
Solanum lycopersicum
Front. Plant Sci.
8
21
2017
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1
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6
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2
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4
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6
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2
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6
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6
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6
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2
4
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-
745906
Misra
Two CYP716A subfamily cytochr ...
Ocimum basilicum
New Phytol.
214
706-720
2017
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-
1
-
1
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-
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4
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4
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3
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5
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3
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1
3
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1
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4
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3
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5
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1
3
3
1
-
-
746004
Jo
beta-Amyrin synthase (EsBAS) ...
Eleutherococcus senticosus
Phytochemistry
135
53-63
2017
-
-
1
-
-
-
-
-
1
1
-
4
-
7
-
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2
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7
1
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3
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1
3
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1
1
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4
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2
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7
1
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1
2
2
1
-
-
746048
Tamura
Cytochrome P450 monooxygenase ...
Platycodon grandiflorus
Plant Cell Physiol.
58
874-884
2017
-
-
1
-
-
-
-
-
-
-
-
4
-
5
-
-
-
-
-
4
-
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11
-
1
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2
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2
4
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4
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8
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11
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2
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2
3
-
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-
746049
Han
Transcriptomic analysis of Ka ...
Kalopanax septemlobus
Plant Cell Physiol.
59
319-330
2017
-
-
1
-
-
-
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-
2
1
-
1
-
7
-
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1
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1
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3
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1
3
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2
1
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1
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-
1
-
1
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-
-
-
-
-
2
2
-
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-
746060
Tamura
CYP716A179 functions as a tri ...
Glycyrrhiza uralensis, Glycyrrhiza uralensis 308-19
Plant Cell Rep.
36
437-445
2017
-
-
1
-
-
-
-
-
-
-
-
16
-
6
-
-
-
-
-
6
-
-
18
-
-
-
-
-
-
-
-
2
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1
2
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16
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6
-
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18
-
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-
748772
Andre
Multifunctional oxidosqualene ...
Malus domestica
New Phytol.
211
1279-1294
2016
-
-
1
-
-
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-
-
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5
-
2
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1
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6
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1
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1
1
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5
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1
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6
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1
1
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732623
Han
The involvement of beta-amyrin ...
Panax ginseng
Plant Cell Physiol.
54
2034-2046
2013
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-
1
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-
-
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1
1
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5
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2
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2
1
1
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1
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1
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1
1
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2
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2
1
1
-
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-
1
-
-
-
1
1
1
1
-
-
732622
Fukushima
CYP716A subfamily members are ...
Medicago truncatula, Vitis vinifera
Plant Cell Physiol.
52
2050-2061
2011
-
-
2
-
-
-
-
-
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7
-
6
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4
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2
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19
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3
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7
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4
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19
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732627
Carelli
Medicago truncatula CYP716A12 ...
Medicago truncatula
Plant Cell
23
3070-3081
2011
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1
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1
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1
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5
-
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5
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2
1
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1
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1
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1
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5
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2
1
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1
1
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