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show all sequences of 1.14.14.127

Expressional and functional analysis of CYP15A1, a juvenile hormone epoxidase, in the red flour beetle Tribolium castaneum

Minakuchi, C.; Ishii, F.; Washidu, Y.; Ichikawa, A.; Tanaka, T.; Miura, K.; Shinoda, T.; J. Insect Physiol. 80, 61-70 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Sf9 cells
Tribolium castaneum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Tribolium castaneum
D6WFY6
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
corpus allatum
-
Tribolium castaneum
-
additional information
level of CYP15A1 mRNA is high in the embryonic stage as well as in the middle of the final larval instar. In the embryonic stage, the transcript level of CYP15A1 starts to increase 30 h after egg laying and peaks 54-60 h after egg laying
Tribolium castaneum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
732291
Tribolium castaneum
? + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
732291
Tribolium castaneum
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
product has (10R)-configuration
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Sf9 cells
Tribolium castaneum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
corpus allatum
-
Tribolium castaneum
-
additional information
level of CYP15A1 mRNA is high in the embryonic stage as well as in the middle of the final larval instar. In the embryonic stage, the transcript level of CYP15A1 starts to increase 30 h after egg laying and peaks 54-60 h after egg laying
Tribolium castaneum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
732291
Tribolium castaneum
? + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
732291
Tribolium castaneum
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
product has (10R)-configuration
-
-
?
General Information
General Information
Commentary
Organism
physiological function
RNAi-mediated knockdown of CYP15A1 in the pre-final larval instar does not result in precocious metamorphosis to pupa. The double knockdown of juvenile hormone acid O-methyltransferase and TcCYP15A1 results in pupae and adults with shorter wings
Tribolium castaneum
General Information (protein specific)
General Information
Commentary
Organism
physiological function
RNAi-mediated knockdown of CYP15A1 in the pre-final larval instar does not result in precocious metamorphosis to pupa. The double knockdown of juvenile hormone acid O-methyltransferase and TcCYP15A1 results in pupae and adults with shorter wings
Tribolium castaneum
Other publictions for EC 1.14.14.127
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745508
Cardoso-Junior
Methyl farnesoate epoxidase ( ...
Melipona scutellaris
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101
185-194
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1
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1
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11
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7
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1
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2
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1
2
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7
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1
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1
1
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746473
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1
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1
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1
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3
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2
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1
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1
1
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1
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2
2
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744987
Jedlicka
Gene expression dynamics in m ...
Bombus terrestris terrestris
Front. Physiol.
7
574
2016
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1
-
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1
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2
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10
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1
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1
1
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1
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10
-
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1
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-
-
-
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-
2
2
-
-
-
732291
Minakuchi
Expressional and functional an ...
Tribolium castaneum
J. Insect Physiol.
80
61-70
2015
-
-
1
-
-
-
-
-
-
-
-
-
-
3
-
-
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3
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2
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1
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3
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2
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1
1
-
-
-
745073
Abrisqueta
Insulin receptor-mediated nut ...
Blattella germanica
Insect Biochem. Mol. Biol.
49
14-23
2014
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
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1
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1
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2
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1
2
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1
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1
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1
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1
2
2
1
-
-
731512
Daimon
Function, diversity, and appli ...
Diploptera punctata
Biotechnol. Appl. Biochem.
60
82-91
2013
-
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1
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1
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1
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732805
Helvig
CYP15A1, the cytochrome P450 t ...
Diploptera punctata
Proc. Natl. Acad. Sci. USA
101
4024-4029
2004
-
1
1
-
-
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4
1
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1
1
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5
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1
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4
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4
1
1
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1
1
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1
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3
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1
1
1
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3
4
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1
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1
1
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1
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4
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4
1
1
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1
1
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731914
Andersen
Photoaffinity labeling of meth ...
Diploptera punctata
Insect Biochem. Mol. Biol.
25
713-719
1995
-
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2
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1
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4
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5
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1
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2
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2
2
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1
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5
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1
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731921
Pratt
-
The influence of a P450 inhibi ...
Periplaneta americana
Insect Biochem.
14
609-614
1984
-
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1
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1
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731740
Feyereisen
Enzymic synthesis of juvenile ...
Locusta migratoria
Eur. J. Biochem.
118
231-238
1981
-
-
-
-
-
-
3
1
1
-
-
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3
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2
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1
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