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Literature summary for 1.14.14.127 extracted from

  • Cardoso-Junior, C.A.M.; Silva, R.P.; Borges, N.A.; de Carvalho, W.J.; Walter, S.L.; Simoes, Z.L.P.; Bitondi, M.M.G.; Ueira Vieira, C.; Bonetti, A.M.; Hartfelder, K.
    Methyl farnesoate epoxidase (mfe) gene expression and juvenile hormone titers in the life cycle of a highly eusocial stingless bee, Melipona scutellaris (2017), J. Insect Physiol., 101, 185-194 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene mfe, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis Melipona scutellaris

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2 Melipona scutellaris
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juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
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?

Organism

Organism UniProt Comment Textmining
Melipona scutellaris A0A286LD89 collected from colonies kept in the Meliponary of the University of Uberlandia, MG Brazil
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Source Tissue

Source Tissue Comment Organism Textmining
adult
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Melipona scutellaris
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corpus allatum gene mfe is exclusively expressed in the corpora allata Melipona scutellaris
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larva
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Melipona scutellaris
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additional information gene mfe expression and juvenile hormone, JH, titers in the life cycle of the highly eusocial stingless bee, hemolymph JH titers for all life cycle stages of Melipona scutellaris queens and workers, overview. The JH titer is high in the second larval instar, drops in the third, and rises again as the larvae enter metamorphosis. During the pupal stage, mfe expression is initialy elevated, but then gradually drops to low levels before adult emergence. No variation is seen in the JH titer. In adult virgin queens, mfe expression and the JH titer are significantly elevated, possibly associated with their reproductive potential. For workers, JH titers are lower in foragers than in nurse bees, while mfe expression does not differ Melipona scutellaris
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pupa
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Melipona scutellaris
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
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Melipona scutellaris juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
?

Synonyms

Synonyms Comment Organism
methylfarnesoate epoxidase
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Melipona scutellaris
MFE
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Melipona scutellaris

Cofactor

Cofactor Comment Organism Structure
cytochrome P450
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Melipona scutellaris
NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Melipona scutellaris

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the final step in juvenile hormone, JH, biosynthesis Melipona scutellaris