BRENDA - Enzyme Database show
show all sequences of 1.14.14.127

Characterization of juvenile hormone related genes regulating cantharidin biosynthesis in Epicauta chinensis

Jiang, M.; Lue, S.; Zhang, Y.; Sci. Rep. 7, 2308 (2017)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
Epicauta chinensis
Engineering
Amino acid exchange
Commentary
Organism
additional information
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection
Epicauta chinensis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158
Epicauta chinensis
16020
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
Epicauta chinensis
-
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Epicauta chinensis
A0A2C9DK12
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
head
expression level of EcMFE is significantly high in the head of males
Epicauta chinensis
-
additional information
temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating
Epicauta chinensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
746473
Epicauta chinensis
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 54810, sequence calculation
Epicauta chinensis
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P450 conserved site is located at amino acids 432-441
Epicauta chinensis
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Epicauta chinensis
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Epicauta chinensis
sequence calculation
-
8.29
Cloned(Commentary) (protein specific)
Commentary
Organism
gene EcMFE, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis
Epicauta chinensis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
cytochrome P450 conserved site is located at amino acids 432-441
Epicauta chinensis
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
Epicauta chinensis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
EcMFE RNAi-mediated knockdown. In EcMFE dsRNA treatment, there are no significant differences between the treated group and controls until the 5th day after injection, when compared on cantharidin content, the cantharidin content is significantly reduced on the 7th day after injection
Epicauta chinensis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
the enzyme has a membrane anchor region at amino acids 2-19, the transmembrane region is located at amino acids 141-158
Epicauta chinensis
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
Epicauta chinensis
-
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
head
expression level of EcMFE is significantly high in the head of males
Epicauta chinensis
-
additional information
temporal and spatial expression patterns of gene EcMFE, relative expression profiles of EcMFE in tissues and developmental stages, overview. The expression level of EcMFE is high in the first instar larval stage. Transcripts of EcMFE, EcJHEH and EcJHAMT show a similar tendency with the cantharidin production in male blister beetles after mating
Epicauta chinensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
methyl (2E,6E)-farnesoate + [reduced NADPH-hemoprotein reductase] + O2
-
746473
Epicauta chinensis
juvenile hormone III + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 54810, sequence calculation
Epicauta chinensis
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Epicauta chinensis
sequence calculation
-
8.29
General Information
General Information
Commentary
Organism
malfunction
interference of gene EcMFE significantly inhibits the biosynthesis of cantharidin in male Epicauta chinensis after mating
Epicauta chinensis
metabolism
juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III
Epicauta chinensis
General Information (protein specific)
General Information
Commentary
Organism
malfunction
interference of gene EcMFE significantly inhibits the biosynthesis of cantharidin in male Epicauta chinensis after mating
Epicauta chinensis
metabolism
juvenile hormone is finally synthesized via methylation and epoxidation regulated by JH acid O-methyltransferase (JHAMT) and methyl farnesoate epoxidase (MFE), respectively. Genes EcMFE and EcJHEH may be involved in the biosynthesis of cantharidin, but juvenile hormone III (JH III) might not be the direct precursor of cantharidin. Cantharidin is a monoterpene that is proposed to be constituted of two isoprene units. Cantharidin is biosynthesized via the mevalonate pathway that can also synthesize JH III
Epicauta chinensis
Other publictions for EC 1.14.14.127
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745508
Cardoso-Junior
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101
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1
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11
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7
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1
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1
1
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746473
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Characterization of juvenile ...
Epicauta chinensis
Sci. Rep.
7
2308
2017
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1
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1
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1
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1
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3
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1
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2
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1
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2
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1
1
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1
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2
2
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744987
Jedlicka
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Bombus terrestris terrestris
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1
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2
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10
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1
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1
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1
1
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1
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10
-
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1
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2
2
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732291
Minakuchi
Expressional and functional an ...
Tribolium castaneum
J. Insect Physiol.
80
61-70
2015
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1
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3
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3
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3
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2
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1
1
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745073
Abrisqueta
Insulin receptor-mediated nut ...
Blattella germanica
Insect Biochem. Mol. Biol.
49
14-23
2014
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-
1
-
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-
-
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1
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3
-
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1
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1
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2
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1
2
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1
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1
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1
2
2
1
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-
731512
Daimon
Function, diversity, and appli ...
Diploptera punctata
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60
82-91
2013
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732805
Helvig
CYP15A1, the cytochrome P450 t ...
Diploptera punctata
Proc. Natl. Acad. Sci. USA
101
4024-4029
2004
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1
1
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4
1
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1
1
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5
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1
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4
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4
1
1
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1
1
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1
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3
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1
1
1
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3
4
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1
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1
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1
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4
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4
1
1
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1
1
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731914
Andersen
Photoaffinity labeling of meth ...
Diploptera punctata
Insect Biochem. Mol. Biol.
25
713-719
1995
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2
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1
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4
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5
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1
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2
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1
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5
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1
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731921
Pratt
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The influence of a P450 inhibi ...
Periplaneta americana
Insect Biochem.
14
609-614
1984
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1
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731740
Feyereisen
Enzymic synthesis of juvenile ...
Locusta migratoria
Eur. J. Biochem.
118
231-238
1981
-
-
-
-
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3
1
1
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3
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2
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