Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.14.129 extracted from

  • Han, J.; Clement, J.M.; Li, J.; King, A.; Ng, S.; Jaworski, J.G.
    The cytochrome P450 CYP86A22 is a fatty acyl-CoA omega-hydroxylase essential for estolide synthesis in the stigma of Petunia hybrida (2010), J. Biol. Chem., 285, 3986-3996.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.129

Organism

Organism UniProt Comment Textmining
Petunia x hybrida B3RFJ6
-
-

Source Tissue

Source Tissue Comment Organism Textmining
stigma expressed predominantly in the developing stigma Petunia x hybrida
-

Storage Stability

Storage Stability Organism
-80°C, stable for at least six months Petunia x hybrida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate preference for both saturated and unsaturated acyl-CoAs rather than free fatty acids. Decreasing substrate preference order in vitro is 16:0-CoA, 18:1-CoA, 18:0-CoA Petunia x hybrida ?
-
 ?
oleate + [reduced NADPH-hemoprotein reductase] + O2 about 6% of the activity with palmitoyl-CoA Petunia x hybrida 18-hydroxyoleate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
oleoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
 Petunia x hybrida 18-hydroxyoleoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
palmitoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
 Petunia x hybrida 16-hydroxypalmitoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
palmitoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
 Petunia x hybrida 16-hydroxypalmitoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O about 21% of the activity with palmitoyl-CoA ?
stearoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
 Petunia x hybrida 18-hydroxystearoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O about 18% of the activity with palmitoyl-CoA ?

Synonyms

Synonyms Comment Organism
CYP86A22
-
 Petunia x hybrida
cytochrome P450 86A22
-
 Petunia x hybrida

General Information

General Information Comment Organism
physiological function gene CYP86A22 encodes a fatty acyl-CoA omega-hydroxylase involved in estolide biosynthesis in the stigma of Petunia hybrida. omega-Hydroxy fatty acids are essentially absent in the stigmas from 18 of 46 CYP86A22-RNAi transgenic plants and have varying levels of suppression in the remaining 28 plants. Lipids in the 18 CYP86A22-RNAi stigmas are predominantly triacylglycerols and diacylglycerols instead of the estolides Petunia x hybrida