BRENDA - Enzyme Database show
show all sequences of 1.14.14.129

The cytochrome P450 CYP86A22 is a fatty acyl-CoA omega-hydroxylase essential for estolide synthesis in the stigma of Petunia hybrida

Han, J.; Clement, J.M.; Li, J.; King, A.; Ng, S.; Jaworski, J.G.; J. Biol. Chem. 285, 3986-3996 (2010)

Data extracted from this reference:

Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Petunia x hybrida
B3RFJ6
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
stigma
expressed predominantly in the developing stigma
Petunia x hybrida
-
Storage Stability
Storage Stability
Organism
-80C, stable for at least six months
Petunia x hybrida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
substrate preference for both saturated and unsaturated acyl-CoAs rather than free fatty acids. Decreasing substrate preference order in vitro is 16:0-CoA, 18:1-CoA, 18:0-CoA
734164
Petunia x hybrida
?
-
-
-
-
oleate + [reduced NADPH-hemoprotein reductase] + O2
about 6% of the activity with palmitoyl-CoA
734164
Petunia x hybrida
18-hydroxyoleate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
oleoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
734164
Petunia x hybrida
18-hydroxyoleoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
734164
Petunia x hybrida
16-hydroxypalmitoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
734164
Petunia x hybrida
16-hydroxypalmitoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
about 21% of the activity with palmitoyl-CoA
-
-
?
stearoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
734164
Petunia x hybrida
18-hydroxystearoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
about 18% of the activity with palmitoyl-CoA
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
stigma
expressed predominantly in the developing stigma
Petunia x hybrida
-
Storage Stability (protein specific)
Storage Stability
Organism
-80C, stable for at least six months
Petunia x hybrida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
substrate preference for both saturated and unsaturated acyl-CoAs rather than free fatty acids. Decreasing substrate preference order in vitro is 16:0-CoA, 18:1-CoA, 18:0-CoA
734164
Petunia x hybrida
?
-
-
-
-
oleate + [reduced NADPH-hemoprotein reductase] + O2
about 6% of the activity with palmitoyl-CoA
734164
Petunia x hybrida
18-hydroxyoleate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
oleoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
734164
Petunia x hybrida
18-hydroxyoleoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
734164
Petunia x hybrida
16-hydroxypalmitoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
palmitoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
734164
Petunia x hybrida
16-hydroxypalmitoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
about 21% of the activity with palmitoyl-CoA
-
-
?
stearoyl-CoA + [reduced NADPH-hemoprotein reductase] + O2
-
734164
Petunia x hybrida
18-hydroxystearoyl-CoA + [oxidized NADPH-hemoprotein reductase] + H2O
about 18% of the activity with palmitoyl-CoA
-
-
?
General Information
General Information
Commentary
Organism
physiological function
gene CYP86A22 encodes a fatty acyl-CoA omega-hydroxylase involved in estolide biosynthesis in the stigma of Petunia hybrida. omega-Hydroxy fatty acids are essentially absent in the stigmas from 18 of 46 CYP86A22-RNAi transgenic plants and have varying levels of suppression in the remaining 28 plants. Lipids in the 18 CYP86A22-RNAi stigmas are predominantly triacylglycerols and diacylglycerols instead of the estolides
Petunia x hybrida
General Information (protein specific)
General Information
Commentary
Organism
physiological function
gene CYP86A22 encodes a fatty acyl-CoA omega-hydroxylase involved in estolide biosynthesis in the stigma of Petunia hybrida. omega-Hydroxy fatty acids are essentially absent in the stigmas from 18 of 46 CYP86A22-RNAi transgenic plants and have varying levels of suppression in the remaining 28 plants. Lipids in the 18 CYP86A22-RNAi stigmas are predominantly triacylglycerols and diacylglycerols instead of the estolides
Petunia x hybrida
Other publictions for EC 1.14.14.129
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
734164
Han
The cytochrome P450 CYP86A22 i ...
Petunia x hybrida
J. Biol. Chem.
285
3986-3996
2010
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