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show all sequences of 1.14.14.135

Induction of phytoalexin synthesis in soybean: enzymatic cyclization of prenylated pterocarpans to glyceollin isomers

Welle, R.; Grisebach, H.; Arch. Biochem. Biophys. 263, 191-198 (1988)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
challenge with either a glucan elicitor from Phytophthora megasperma f.sp. glycinea or with yeast extract causes strong stimulation of cyclase activity with a maximum at about 24 h after elicitor addition
Glycine max
NADH
a synergistic effect of NADH, 94% increase in cyclase activity, is observed at low NADPH concentrations of about 0.01 mM
Glycine max
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ancymidol
0.01 mM: 15% inhibition, 0.1 mM: 85% inhibition
Glycine max
BAS 110
0.1 mM, 15% inhibition
Glycine max
BAS 111
0.1 mM, 39% inhibition
Glycine max
CO
inhibits in a white light-reversible manner in the presence of oxygen
Glycine max
cytochrome c
0.01 mM: 25% inhibition, 0.025 mM: 64% inhibition, 0.05 mM: 85% inhibition
Glycine max
glyceollin
product inhibition, 0.058 mM, 50% inhibition
Glycine max
ketoconazol
0.01 mM: 34% inhibition, 0.1 mM: 100% inhibition
Glycine max
LAB 978
0.01 mM: 17% inhibition, 0.1 mM: 49% inhibition
Glycine max
Metyrapone
0.01 mM: 15% inhibition, 0.1 mM: 37% inhibition
Glycine max
NADP+
strong inhibition by NADP+ at low NADPH concentrations
Glycine max
potassium isothiocyanate
0.01 mM: 19% inhibition, 0.1 mM: 73% inhibition, 1 mM: 100% inhibition
Glycine max
SKF 525
0.1 mM: 11% inhibition, 1 mM: 100% inhibition
Glycine max
tetcyclasis
0.01 mM: 9% inhibition, 0.1 mM: 67% inhibition
Glycine max
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
2-(or 4-)dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan
pH 7.5, 20C, mixture of the two glyceollidin isomers
Glycine max
0.03
-
perrhenate
pH 7.5, 20C
Glycine max
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
the cyclase is associated with
Glycine max
5783
-
microsome
-
Glycine max
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Glycine max
last committed step in the glyceollin biosynthesis
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycine max
-
cv. Harosoy 63
-
Reaction
Reaction
Commentary
Organism
2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2 = glyceollin II + [oxidized NADPH-hemoprotein reductase] + 2 H2O
a heme-thiolate protein, P-450, glyceollins II and III are formed from 2-dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan whereas glyceollin I is formed from the 4-isomer
Glycine max
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
elicitor-challenged, unstimulated cell culture does not contain detectable cyclase activity
Glycine max
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Glycine max
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(or 4-)dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
enzyme catalyzes an NADPH-dependent and oxygen-dependent cyclization of a mixture of 2- and 4-dimethylallylglycinols to the glyceollin isomers I-III
654324
Glycine max
glyceollin + [oxidized NADPH-hemoprotein reductase] + H2O
glyceollin isomers I-III, glyceollin isomer I is formed from 4-dimethylallylglycinol and glyceollin isomers II and III from 2-dimethylallylglycinol
-
-
?
additional information
last committed step in the glyceollin biosynthesis
654324
Glycine max
?
-
-
-
-
additional information
not: NADH, enzyme does not catalyze the conversion of rot-2-enoic acid to deguelin in the presence or absence of NADPH
654324
Glycine max
?
-
-
-
-
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
assay at
Glycine max
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
9.5
very broad pH-optimum
Glycine max
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
pH 7: 50% of maximum activity, no activity measurable above pH 9.5, probably because of the instability of the substrate
Glycine max
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome b
average content of b-type cytochromes: 0.26 nmol/mg protein
Glycine max
cytochrome P-450
cytochrome P-450-dependent monooxygenase, average content of cytochrome P-450: 0.16 nmol/mg protein
Glycine max
O2
oxygen-dependent
Glycine max
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
challenge with either a glucan elicitor from Phytophthora megasperma f.sp. glycinea or with yeast extract causes strong stimulation of cyclase activity with a maximum at about 24 h after elicitor addition
Glycine max
NADH
a synergistic effect of NADH, 94% increase in cyclase activity, is observed at low NADPH concentrations of about 0.01 mM
Glycine max
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome b
average content of b-type cytochromes: 0.26 nmol/mg protein
Glycine max
cytochrome P-450
cytochrome P-450-dependent monooxygenase, average content of cytochrome P-450: 0.16 nmol/mg protein
Glycine max
O2
oxygen-dependent
Glycine max
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ancymidol
0.01 mM: 15% inhibition, 0.1 mM: 85% inhibition
Glycine max
BAS 110
0.1 mM, 15% inhibition
Glycine max
BAS 111
0.1 mM, 39% inhibition
Glycine max
CO
inhibits in a white light-reversible manner in the presence of oxygen
Glycine max
cytochrome c
0.01 mM: 25% inhibition, 0.025 mM: 64% inhibition, 0.05 mM: 85% inhibition
Glycine max
glyceollin
product inhibition, 0.058 mM, 50% inhibition
Glycine max
ketoconazol
0.01 mM: 34% inhibition, 0.1 mM: 100% inhibition
Glycine max
LAB 978
0.01 mM: 17% inhibition, 0.1 mM: 49% inhibition
Glycine max
Metyrapone
0.01 mM: 15% inhibition, 0.1 mM: 37% inhibition
Glycine max
NADP+
strong inhibition by NADP+ at low NADPH concentrations
Glycine max
potassium isothiocyanate
0.01 mM: 19% inhibition, 0.1 mM: 73% inhibition, 1 mM: 100% inhibition
Glycine max
SKF 525
0.1 mM: 11% inhibition, 1 mM: 100% inhibition
Glycine max
tetcyclasis
0.01 mM: 9% inhibition, 0.1 mM: 67% inhibition
Glycine max
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.002
-
2-(or 4-)dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan
pH 7.5, 20C, mixture of the two glyceollidin isomers
Glycine max
0.03
-
perrhenate
pH 7.5, 20C
Glycine max
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
the cyclase is associated with
Glycine max
5783
-
microsome
-
Glycine max
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Glycine max
last committed step in the glyceollin biosynthesis
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
elicitor-challenged, unstimulated cell culture does not contain detectable cyclase activity
Glycine max
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
-
Glycine max
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-(or 4-)dimethylallyl-(6aS,11aS)-3,6a,9-trihydroxypterocarpan + [reduced NADPH-hemoprotein reductase] + O2
enzyme catalyzes an NADPH-dependent and oxygen-dependent cyclization of a mixture of 2- and 4-dimethylallylglycinols to the glyceollin isomers I-III
654324
Glycine max
glyceollin + [oxidized NADPH-hemoprotein reductase] + H2O
glyceollin isomers I-III, glyceollin isomer I is formed from 4-dimethylallylglycinol and glyceollin isomers II and III from 2-dimethylallylglycinol
-
-
?
additional information
last committed step in the glyceollin biosynthesis
654324
Glycine max
?
-
-
-
-
additional information
not: NADH, enzyme does not catalyze the conversion of rot-2-enoic acid to deguelin in the presence or absence of NADPH
654324
Glycine max
?
-
-
-
-
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
20
-
assay at
Glycine max
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
9.5
very broad pH-optimum
Glycine max
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9.5
pH 7: 50% of maximum activity, no activity measurable above pH 9.5, probably because of the instability of the substrate
Glycine max
Other publictions for EC 1.14.14.135
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
654324
Welle
Induction of phytoalexin synth ...
Glycine max
Arch. Biochem. Biophys.
263
191-198
1988
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