BRENDA - Enzyme Database show
show all sequences of 1.14.14.141

Elicitor-induced biosynthesis of psoralens in Ammi majus L. suspension cultures. Microsomal conversion of demethylsuberosin into (+)marmesin and psoralen

Hamerski, D.; Matern, U.; Eur. J. Biochem. 171, 369-375 (1988)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
additional information
elicitor preparations from either Alternaria carthami Chowdhury or Phytophthora megasperma f.sp. glycinea induce the production of linear furanocoumarins in Ammi majus suspension culture
Ammi majus
Inhibitors
Inhibitors
Commentary
Organism
Structure
Ancymidole
13% inhibition of the psoralen synthase at 0.05 mM
Ammi majus
BAS 110
72% inhibition of the psoralen synthase at 0.05 mM, 38% at 0.005 mM
Ammi majus
BAS 111
complete inhibition of the psoralen synthase at 0.001 mM, 86% at 0.0002 mM
Ammi majus
BAS 978
complete inhibition of the psoralen synthase at 0.05 mM, 68% at 0.005 mM
Ammi majus
ketoconazole
complete inhibition of the psoralen synthase at 0.001 mM, 74% at 0.0002 mM
Ammi majus
Tetcyclacis
85% inhibition of the psoralen synthase at 0.001 mM, 25% at 0.0002 mM
Ammi majus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum lumen
-
Ammi majus
5788
-
microsome
-
Ammi majus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
Ammi majus
the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
?
additional information
Ammi majus
umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Ammi majus
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme partially by preparation of microsomes
Ammi majus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Ammi majus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
673426
Ammi majus
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
-
?
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium
673426
Ammi majus
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
-
?
additional information
umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin
673426
Ammi majus
?
-
-
-
-
additional information
the psoralen synthase is a cytochrome-P450-dependent monooxygenase
673426
Ammi majus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
assay at
Ammi majus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Ammi majus
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the psoralen synthase is a cytochrome-P450-dependent monooxygenase
Ammi majus
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
additional information
elicitor preparations from either Alternaria carthami Chowdhury or Phytophthora megasperma f.sp. glycinea induce the production of linear furanocoumarins in Ammi majus suspension culture
Ammi majus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the psoralen synthase is a cytochrome-P450-dependent monooxygenase
Ammi majus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Ancymidole
13% inhibition of the psoralen synthase at 0.05 mM
Ammi majus
BAS 110
72% inhibition of the psoralen synthase at 0.05 mM, 38% at 0.005 mM
Ammi majus
BAS 111
complete inhibition of the psoralen synthase at 0.001 mM, 86% at 0.0002 mM
Ammi majus
BAS 978
complete inhibition of the psoralen synthase at 0.05 mM, 68% at 0.005 mM
Ammi majus
ketoconazole
complete inhibition of the psoralen synthase at 0.001 mM, 74% at 0.0002 mM
Ammi majus
Tetcyclacis
85% inhibition of the psoralen synthase at 0.001 mM, 25% at 0.0002 mM
Ammi majus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum lumen
-
Ammi majus
5788
-
microsome
-
Ammi majus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
Ammi majus
the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
?
additional information
Ammi majus
umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme partially by preparation of microsomes
Ammi majus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Ammi majus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
-
673426
Ammi majus
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
-
?
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2
the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium
673426
Ammi majus
psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O
-
-
-
?
additional information
umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin
673426
Ammi majus
?
-
-
-
-
additional information
the psoralen synthase is a cytochrome-P450-dependent monooxygenase
673426
Ammi majus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
20
-
assay at
Ammi majus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at
Ammi majus
Other publictions for EC 1.14.14.141
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
728497
Parast
In vitro isolation, elicitatio ...
Cullen corylifolium
Plant Physiol. Biochem.
49
1138-1146
2011
-
-
1
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
6
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
698971
Larbat
Isolation and functional chara ...
Apium graveolens, Pastinaca sativa
J. Biol. Chem.
284
4776-4785
2009
-
-
2
-
-
-
-
4
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
4
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
674887
Larbat
Molecular cloning and function ...
Ammi majus
J. Biol. Chem.
282
542-554
2007
1
-
1
-
1
-
2
1
1
1
1
2
-
6
-
-
1
1
-
2
-
-
4
2
1
-
-
1
1
-
-
1
-
1
-
1
-
1
1
-
1
-
-
2
-
1
1
1
1
2
-
-
-
1
-
2
-
-
4
2
1
-
-
1
1
-
-
1
-
-
-
-
-
-
673426
Hamerski
Elicitor-induced biosynthesis ...
Ammi majus
Eur. J. Biochem.
171
369-375
1988
1
-
-
-
-
-
6
-
2
-
-
2
-
4
-
-
1
-
-
1
-
-
4
-
1
-
-
-
1
-
-
1
-
-
-
1
-
-
1
-
-
-
-
6
-
-
2
-
-
2
-
-
-
1
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
673425
Wendorff
Differential response of cultu ...
Petroselinum crispum
Eur. J. Biochem.
161
391-398
1986
2
-
-
-
-
-
10
1
2
1
-
1
-
5
-
-
1
1
-
1
-
-
3
-
1
-
1
-
1
-
-
1
-
-
-
2
-
-
1
-
-
-
-
10
-
1
2
1
-
1
-
-
-
1
-
1
-
-
3
-
1
-
1
-
1
-
-
-
-
-
-
-
-
-