Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | elicitor preparations from either Alternaria carthami Chowdhury or Phytophthora megasperma f.sp. glycinea induce the production of linear furanocoumarins in Ammi majus suspension culture | Ammi majus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ancymidole | 13% inhibition of the psoralen synthase at 0.05 mM | Ammi majus | |
BAS 110 | 72% inhibition of the psoralen synthase at 0.05 mM, 38% at 0.005 mM | Ammi majus | |
BAS 111 | complete inhibition of the psoralen synthase at 0.001 mM, 86% at 0.0002 mM | Ammi majus | |
BAS 978 | complete inhibition of the psoralen synthase at 0.05 mM, 68% at 0.005 mM | Ammi majus | |
ketoconazole | complete inhibition of the psoralen synthase at 0.001 mM, 74% at 0.0002 mM | Ammi majus | |
Tetcyclacis | 85% inhibition of the psoralen synthase at 0.001 mM, 25% at 0.0002 mM | Ammi majus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum lumen | - |
Ammi majus | 5788 | - |
microsome | - |
Ammi majus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 | Ammi majus | the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium | psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O | - |
? | |
additional information | Ammi majus | umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ammi majus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme partially by preparation of microsomes | Ammi majus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Ammi majus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Ammi majus | psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O | - |
? | |
(+)-marmesin + [reduced NADPH-hemoprotein reductase] + O2 | the substrate is synthesized from demethylsuberosin by the marmesin synthase, biosynthesis of furanocoumarin phytoalexin which are then excreted from the endoplasmic reticulum lumen into the cell culture medium | Ammi majus | psoralen + [oxidized NADPH-hemoprotein reductase] + acetone + 2 H2O | - |
? | |
additional information | umbelliferone is a better precursor of psoralens than either demethylsuberosin or marmesin | Ammi majus | ? | - |
? | |
additional information | the psoralen synthase is a cytochrome-P450-dependent monooxygenase | Ammi majus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Ammi majus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Ammi majus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the psoralen synthase is a cytochrome-P450-dependent monooxygenase | Ammi majus |