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show all sequences of 1.14.14.142

Characterization of leachianone G 2''-dimethylallyltransferase, a novel prenyl side-chain elongation enzyme for the formation of the lavandulyl group of sophoraflavanone G in Sophora flavescens Ait. cell suspension cultures

Zhao, P.; Inoue, K.; Kouno, I.; Yamamoto, H.; Plant Physiol. 133, 1306-1313 (2003)

Data extracted from this reference:

Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Sophora flavescens
5783
-
additional information
subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview
Sophora flavescens
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
Sophora flavescens
the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Sophora flavescens
-
-
-
Purification (Commentary)
Commentary
Organism
native enzyme partially by microsome preparation
Sophora flavescens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Sophora flavescens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
-
676566
Sophora flavescens
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview
676566
Sophora flavescens
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
the enzyme is a cytochrome P450 monooxygenase
Sophora flavescens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
the enzyme is a cytochrome P450 monooxygenase
Sophora flavescens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Sophora flavescens
5783
-
additional information
subcellular localization of the enzymes involved in the biosynthetic route from naringenin to sophoraflavanone G, overview
Sophora flavescens
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
Sophora flavescens
the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme partially by microsome preparation
Sophora flavescens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
cell suspension culture
-
Sophora flavescens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
-
676566
Sophora flavescens
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
8-dimethylallylnaringenin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is crucial for lavandulylated flavanone formation, the enzyme catalyzes the 2'-hydroxylation of 8-DMAN, which is then formed to sophoraflavanone G, pathway overview
676566
Sophora flavescens
leachianone G + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.142
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
676566
Zhao
Characterization of leachianon ...
Sophora flavescens
Plant Physiol.
133
1306-1313
2003
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2
-
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1
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3
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1
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1
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2
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-
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1
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1
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2
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1
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1
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1
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2
-
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676359
Yamamoto
8-dimethylallylnaringenin 2'-h ...
Sophora flavescens
Phytochemistry
58
671-676
2001
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-
-
-
-
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6
1
1
-
-
1
-
2
-
-
-
-
-
1
-
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3
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
6
-
1
1
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
1
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-
-
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-
-
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