BRENDA - Enzyme Database show
show all sequences of 1.14.14.143

Demonstration that menthofuran synthase of mint (Mentha) is a cytochrome P450 monooxygenase: cloning, functional expression, and characterization of the responsible gene

Bertea, C.M.; Schalk, M.; Karp, F.; Maffei, M.; Croteau, R.; Arch. Biochem. Biophys. 390, 279-286 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
DNA and amino acid sequence determinationand analysis, functional expression in Saccharomyces cerevisiae strains WAT11U and WAT21U, which constitutively express two different isoforms of the Arabidposis thaliana NADPH-cytochrome P450 reductase, and in Escherichia coli strain JM109
Mentha pulegium
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Mentha pulegium
-
-
microsome
-
Mentha x piperita
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
-
Mentha pulegium
Fe2+
-
Mentha x piperita
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55360
-
x * 55360, sequence calculation
Mentha pulegium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
Mentha x piperita
-
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
Mentha pulegium
-
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mentha pulegium
-
-
-
Mentha x piperita
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Mentha pulegium
-
leaf
-
Mentha x piperita
-
plant oil gland
secretory cells
Mentha pulegium
-
plant oil gland
secretory cells
Mentha x piperita
-
secretory cell
-
Mentha pulegium
-
secretory cell
-
Mentha x piperita
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
-
684661
Mentha x piperita
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
-
684661
Mentha pulegium
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation of the syn-methyl group of (+)-pulegone, spontaneous intramolecular cyclization to the hemiketal, and dehydration to the furan
684661
Mentha x piperita
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation of the syn-methyl group of (+)-pulegone, spontaneous intramolecular cyclization to the hemiketal, and dehydration to the furan
684661
Mentha pulegium
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
GC-MS product analysis
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 55360, sequence calculation
Mentha pulegium
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Mentha pulegium
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Mentha pulegium
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
a cytochrome P450 monooxygenase
Mentha pulegium
cytochrome P450
a cytochrome P450 monooxygenase
Mentha x piperita
FAD
-
Mentha pulegium
FMN
-
Mentha pulegium
Cloned(Commentary) (protein specific)
Commentary
Organism
DNA and amino acid sequence determinationand analysis, functional expression in Saccharomyces cerevisiae strains WAT11U and WAT21U, which constitutively express two different isoforms of the Arabidposis thaliana NADPH-cytochrome P450 reductase, and in Escherichia coli strain JM109
Mentha pulegium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
a cytochrome P450 monooxygenase
Mentha pulegium
cytochrome P450
a cytochrome P450 monooxygenase
Mentha x piperita
FAD
-
Mentha pulegium
FMN
-
Mentha pulegium
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Mentha pulegium
-
-
microsome
-
Mentha x piperita
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
-
Mentha pulegium
Fe2+
-
Mentha x piperita
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
55360
-
x * 55360, sequence calculation
Mentha pulegium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
Mentha x piperita
-
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
Mentha pulegium
-
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Mentha pulegium
-
leaf
-
Mentha x piperita
-
plant oil gland
secretory cells
Mentha pulegium
-
plant oil gland
secretory cells
Mentha x piperita
-
secretory cell
-
Mentha pulegium
-
secretory cell
-
Mentha x piperita
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
-
684661
Mentha x piperita
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
-
684661
Mentha pulegium
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation of the syn-methyl group of (+)-pulegone, spontaneous intramolecular cyclization to the hemiketal, and dehydration to the furan
684661
Mentha x piperita
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
(+)-pulegone + [reduced NADPH-hemoprotein reductase] + O2
hydroxylation of the syn-methyl group of (+)-pulegone, spontaneous intramolecular cyclization to the hemiketal, and dehydration to the furan
684661
Mentha pulegium
(+)-menthofuran + [oxidized NADPH-hemoprotein reductase] + H2O
GC-MS product analysis
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 55360, sequence calculation
Mentha pulegium
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
30
-
assay at
Mentha pulegium
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Mentha pulegium
Other publictions for EC 1.14.14.143
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746129
Nazari
Physiological, biochemical an ...
Mentha x piperita
Plant Physiol. Biochem.
120
202-212
2017
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1
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1
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1
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1
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1
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1
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2
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1
2
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1
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1
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1
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1
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1
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1
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745751
Kumar
-
Development and characterizat ...
Mentha x piperita, no activity in Mentha arvensis cv. Kosi
Mol. Breed.
34
717-724
2014
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1
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1
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1
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2
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3
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1
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2
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1
2
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1
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1
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3
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1
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1
1
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728548
Atsbaha Zebelo
Chrysolina herbacea modulates ...
Mentha aquatica
PLoS ONE
6
e17195
2011
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1
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6
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1
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728665
Lange
Improving peppermint essential ...
Mentha x piperita
Proc. Natl. Acad. Sci. USA
108
16944-16949
2011
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1
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2
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1
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689735
Mahmoud
Menthofuran regulates essentia ...
Mentha x piperita
Proc. Natl. Acad. Sci. USA
100
14481-14486
2003
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1
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1
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1
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3
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1
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1
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1
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286420
Mahmoud
Metabolic engineering of essen ...
Mentha x piperita
Proc. Natl. Acad. Sci. USA
98
8915-8920
2001
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1
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1
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1
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1
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3
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1
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1
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1
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1
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1
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1
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2
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684661
Bertea
Demonstration that menthofuran ...
Mentha pulegium, Mentha x piperita
Arch. Biochem. Biophys.
390
279-286
2001
-
-
1
-
-
-
-
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2
2
1
2
-
7
-
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6
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4
1
1
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1
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4
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1
4
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2
2
1
2
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6
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4
1
1
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1
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