BRENDA - Enzyme Database show
show all sequences of 1.14.14.146

Improvement of thin-layer chromatography for enzyme assay of geranylgeraniol 18-hydroxylase from Croton stellatopilosus Ohba

Chanama, M.; Wunnakup, T.; De-Eknamkul, W.; Chanama, S.; J. Planar Chromatogr. 22, 49-53 (2009)
No PubMed abstract available

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
Croton stellatopilosus
last step of plaunotol biosynthesis
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
Croton stellatopilosus Ohba
last step of plaunotol biosynthesis
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Croton stellatopilosus
-
Ohba
-
Croton stellatopilosus Ohba
-
Ohba
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Croton stellatopilosus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
improvement of thin-layer chromatography for enzyme assay of geranylgeraniol 18-hydroxylase
Croton stellatopilosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
last step of plaunotol biosynthesis
693849
Croton stellatopilosus
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
last step of plaunotol biosynthesis
693849
Croton stellatopilosus Ohba
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Croton stellatopilosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Croton stellatopilosus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
Croton stellatopilosus
last step of plaunotol biosynthesis
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
Croton stellatopilosus Ohba
last step of plaunotol biosynthesis
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Croton stellatopilosus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
improvement of thin-layer chromatography for enzyme assay of geranylgeraniol 18-hydroxylase
Croton stellatopilosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
last step of plaunotol biosynthesis
693849
Croton stellatopilosus
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
geranylgeraniol + [reduced NADPH-hemoprotein reductase] + O2
last step of plaunotol biosynthesis
693849
Croton stellatopilosus Ohba
18-hydroxygeranylgeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Croton stellatopilosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Croton stellatopilosus
Other publictions for EC 1.14.14.146
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
693849
Chanama
-
Improvement of thin-layer chro ...
Croton stellatopilosus, Croton stellatopilosus Ohba
J. Planar Chromatogr.
22
49-53
2009
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2
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1
1
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1
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2
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1
1
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2
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1
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1
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-
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706809
Battilana
The 1-deoxy-D: -xylulose 5-pho ...
Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis vinifera
Theor. Appl. Genet.
118
653-669
2009
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2
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2
2
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694596
Tansakul
-
Geranylgeraniol-18-hydroxylase ...
Croton sublyratus
Phytochemistry
47
1241-1246
1998
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1
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1
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1
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3
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1
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1
1
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1
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1
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3
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1
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1
1
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