BRENDA - Enzyme Database show
show all sequences of 1.14.14.148

Isolation and functional characterization of CYP71AJ4 encoding for the first P450 monooxygenase of angular furanocoumarin biosynthesis

Larbat, R.; Hehn, A.; Hans, J.; Schneider, S.; Jugde, H.; Schneider, B.; Matern, U.; Bourgaud, F.; J. Biol. Chem. 284, 4776-4785 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in yeast
Pastinaca sativa
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
(+)-columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
0.0014
-
syn-[3'D]columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
0.002
-
syn-[3'D]columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
0.0021
-
(+)-columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
0.0038
-
syn-[3'D]columbianetin
pH 7.0, anti-3'-hydroxylation of substrate
Pastinaca sativa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2
Pastinaca sativa
the enzyme is involved in angular furanocoumarin pathway
angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
negligible amounts of a hydroxylated columbianetin by-product
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pastinaca sativa
C0SJS3
fragment
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Pastinaca sativa
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in angular furanocoumarin pathway
698971
Pastinaca sativa
angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
negligible amounts of a hydroxylated columbianetin by-product
-
-
?
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2
angelicin synthase attacks columbianetin by syn-elimination of hydrogen from C-3'
698971
Pastinaca sativa
angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
negligible amounts of a hydroxylated columbianetin by-product
-
-
?
syn-[3'D]columbianetin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Pastinaca sativa
syn-[3'D](anti-3'-hydroxy)-columbianetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
30
-
Pastinaca sativa
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.06
-
syn-[3'D]columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
0.27
-
syn-[3'D]columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
0.32
-
(+)-columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
1.9
-
(+)-columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
3.9
-
syn-[3'D]columbianetin
pH 7.0, anti-3'-hydroxylation of substrate
Pastinaca sativa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pastinaca sativa
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Pastinaca sativa
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in yeast
Pastinaca sativa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Pastinaca sativa
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0014
-
(+)-columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
0.0014
-
syn-[3'D]columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
0.002
-
syn-[3'D]columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
0.0021
-
(+)-columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
0.0038
-
syn-[3'D]columbianetin
pH 7.0, anti-3'-hydroxylation of substrate
Pastinaca sativa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2
Pastinaca sativa
the enzyme is involved in angular furanocoumarin pathway
angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
negligible amounts of a hydroxylated columbianetin by-product
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Pastinaca sativa
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2
the enzyme is involved in angular furanocoumarin pathway
698971
Pastinaca sativa
angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
negligible amounts of a hydroxylated columbianetin by-product
-
-
?
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2
angelicin synthase attacks columbianetin by syn-elimination of hydrogen from C-3'
698971
Pastinaca sativa
angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
negligible amounts of a hydroxylated columbianetin by-product
-
-
?
syn-[3'D]columbianetin + [reduced NADPH-hemoprotein reductase] + O2
-
698971
Pastinaca sativa
syn-[3'D](anti-3'-hydroxy)-columbianetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
27
30
-
Pastinaca sativa
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.06
-
syn-[3'D]columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
0.27
-
syn-[3'D]columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
0.32
-
(+)-columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
1.9
-
(+)-columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
3.9
-
syn-[3'D]columbianetin
pH 7.0, anti-3'-hydroxylation of substrate
Pastinaca sativa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
-
Pastinaca sativa
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
30
-
syn-[3'D]columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
188
-
syn-[3'D]columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
227
-
(+)-columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
920
-
(+)-columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
967
-
syn-[3'D]columbianetin
pH 7.0, anti-3'-hydroxylation of substrate
Pastinaca sativa
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
30
-
syn-[3'D]columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
188
-
syn-[3'D]columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
227
-
(+)-columbianetin
pH 7.0, isopropyloxy-hydroxylation of substrate
Pastinaca sativa
920
-
(+)-columbianetin
pH 7.0, formation of angelicin
Pastinaca sativa
967
-
syn-[3'D]columbianetin
pH 7.0, anti-3'-hydroxylation of substrate
Pastinaca sativa
Other publictions for EC 1.14.14.148
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
698971
Larbat
Isolation and functional chara ...
Pastinaca sativa
J. Biol. Chem.
284
4776-4785
2009
-
-
1
-
-
-
-
5
-
-
-
1
-
5
-
-
-
-
-
1
-
-
3
-
1
-
-
5
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
5
-
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
5
1
-
-
-
-
-
-
-
5
5