BRENDA - Enzyme Database show
show all sequences of 1.14.14.149

Probing sesquiterpene hydroxylase activities in a coupled assay with terpene synthases

Greenhagen, B.T.; Griggs, P.; Takahashi, S.; Ralston, L.; Chappell, J.; Arch. Biochem. Biophys. 409, 385-394 (2003)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in a WAT11 line of yeast
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
3-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
3-deoxycapsidiol is a reaction product when EAH is incubated with high concentrations (above 0.4 mM) of the 5-epiaristolochene substrate
3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
1-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
premnaspirodiene + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
solavetivone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
Q94FM7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
3-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
3-deoxycapsidiol is a reaction product when EAH is incubated with high concentrations (above 0.4 mM) of the 5-epiaristolochene substrate
710995
Nicotiana tabacum
3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
EAH mediates regio- and stereospecific hydroxylations at the C1 and C3 positions of 5-epi-aristolochene
710995
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
1-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
premnaspirodiene + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
solavetivone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
heme
-
Nicotiana tabacum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in a WAT11 line of yeast
Nicotiana tabacum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
-
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
3-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
3-deoxycapsidiol is a reaction product when EAH is incubated with high concentrations (above 0.4 mM) of the 5-epiaristolochene substrate
3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
1-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
premnaspirodiene + [reduced NADPH-hemoprotein reductase] + O2
Nicotiana tabacum
-
solavetivone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
3-deoxycapsidiol + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
3-deoxycapsidiol is a reaction product when EAH is incubated with high concentrations (above 0.4 mM) of the 5-epiaristolochene substrate
710995
Nicotiana tabacum
3-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
EAH mediates regio- and stereospecific hydroxylations at the C1 and C3 positions of 5-epi-aristolochene
710995
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
1-deoxycapsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
premnaspirodiene + [reduced NADPH-hemoprotein reductase] + O2
-
710995
Nicotiana tabacum
solavetivone + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745907
Lee
Expansion of sesquiterpene bi ...
Capsicum annuum
New Phytol.
215
1132-1143
2017
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
712358
Takahashi
Kinetic and molecular analysis ...
Nicotiana tabacum
J. Biol. Chem.
280
3686-3696
2005
1
-
1
-
7
-
1
10
-
-
-
-
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
10
-
-
-
1
-
-
-
1
-
1
1
-
7
-
-
1
-
10
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
10
-
-
-
-
-
-
-
-
10
10
710995
Greenhagen
Probing sesquiterpene hydroxyl ...
Nicotiana tabacum
Arch. Biochem. Biophys.
409
385-394
2003
-
-
1
-
-
-
-
-
-
-
-
6
-
2
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
710993
Ralston
Cloning, heterologous expressi ...
Nicotiana tabacum
Arch. Biochem. Biophys.
393
222-235
2001
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
1
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-