BRENDA - Enzyme Database show
show all sequences of 1.14.14.149

Kinetic and molecular analysis of 5-epiaristolochene 1,3-dihydroxylase, a cytochrome P450 enzyme catalyzing successive hydroxylations of sesquiterpenes

Takahashi, S.; Zhao, Y.; O'Maille, P.E.; Greenhagen, B.T.; Noel, J.P.; Coates, R.M.; Chappell, J.; J. Biol. Chem. 280, 3686-3696 (2005)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
dimethyl sulfoxide
maximum conversion of 5-epiaristolochene to capsidiol activity is observed at final concentrations of 2-5% (v/v) dimethyl sulfoxide
Nicotiana tabacum
Cloned(Commentary)
Commentary
Organism
expressed in WAT11 yeast strain
Nicotiana tabacum
Engineering
Amino acid exchange
Commentary
Organism
I468A
the mutant produces significant amounts 1beta-hydroxy-5-epiaristolochene, but negligible amounts of capsidiol from 5-epiaristolochene
Nicotiana tabacum
S368A
the mutant retains its ability to fully convert 5-epiaristolochene to capsidiol, but the turnover rates for capsidiol formation is 3-13times lower compared with that of the wild type enzyme and is also able to convert 1beta-hydroxy-5-epiaristolochene to capsidiol with kcat values comparable with that of wild type enzyme, but with Km values for 1beta-hydroxy-5-epiaristolochene 4-6times greater compared with that of wild type
Nicotiana tabacum
S368C
the mutant exhibits wild type catalytic efficiency for 1beta-hydroxy-5-epiaristolochene biosynthesis, but is devoid of the successive hydroxylation activity for capsidiol biosynthesis
Nicotiana tabacum
S368F
devoid of any hydroxylase activity for 5-epiaristolochene or 1beta-hydroxy-5-epiaristolochene
Nicotiana tabacum
S368I
devoid of any hydroxylase activity for 5-epiaristolochene or 1beta-hydroxy-5-epiaristolochene
Nicotiana tabacum
S368T
the mutant retains its ability to fully convert 5-epiaristolochene to capsidiol, but the turnover rates for capsidiol formation is 3-13times lower compared with that of the wild type enzyme and is also able to convert 1beta-hydroxy-5-epiaristolochene to capsidiol with kcat values comparable with that of wild type enzyme, but with Km values for 1beta-hydroxy-5-epiaristolochene 4-6times greater compared with that of wild type
Nicotiana tabacum
S368V
the mutant exhibits wild type catalytic efficiency for 1beta-hydroxy-5-epiaristolochene biosynthesis, but is devoid of the successive hydroxylation activity for capsidiol biosynthesis, the mutant catalyzes the relative equal biosynthesis of 1beta-hydroxy-5-epiaristolochene, 2beta-hydroxy-5-epiaristolochene, and 3beta-hydroxy-5-epiaristolochene from 5-epiaristolochene with wild type efficiency and converts about 1.5% of these monohydroxylated products to their respective ketone forms
Nicotiana tabacum
Inhibitors
Inhibitors
Commentary
Organism
Structure
dimethyl sulfoxide
conversion of 5-epiaristolochene to capsidiol activity is inhibited at concentrations above 10% (v/v) dimethyl sulfoxide
Nicotiana tabacum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00174
-
1beta-hydroxy-5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.0062
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.00997
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.01518
-
5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.01672
-
5-epiaristolochene
mutant enzyme S386V, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.01918
-
5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.02124
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme I486A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.02267
-
5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.03863
-
5-epiaristolochene
mutant enzyme S386C, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.0688
-
5-epiaristolochene
mutant enzyme I486A, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
Q94FM7
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1beta-hydroxy-5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
the catalytic efficiency for 1beta-hydroxy-5-epiaristolochene is about 10times greater than that for 3alpha-hydroxy-5-epiaristolochene
712358
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
3alpha-hydroxy-5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
the catalytic efficiency for 3alpha-hydroxy-5-epiaristolochene is about 10times lower than that for 1beta-hydroxy-5-epiaristolochene
712358
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
712358
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
the release of an 1beta-hydroxy-5-epiaristolochene intermediate at high 5-epiaristolochene concentrations and a 10fold catalytic preference for 1beta-hydroxy-5-epiaristolochene versus 3alpha-hydroxy-5-epiaristolochene is indicative of a preferred reaction order of hydroxylation at C-1, followed by that at C-3
712358
Nicotiana tabacum
1beta-hydroxy-5-epiaristolochene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.037
-
5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.132
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme I486A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.154
-
5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.278
-
5-epiaristolochene
mutant enzyme S386C, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.293
-
5-epiaristolochene
mutant enzyme I486A, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.482
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.493
-
5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.553
-
5-epiaristolochene
mutant enzyme S386V, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.581
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.582
-
1beta-hydroxy-5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
Cofactor
Cofactor
Commentary
Organism
Structure
heme
-
Nicotiana tabacum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
dimethyl sulfoxide
maximum conversion of 5-epiaristolochene to capsidiol activity is observed at final concentrations of 2-5% (v/v) dimethyl sulfoxide
Nicotiana tabacum
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in WAT11 yeast strain
Nicotiana tabacum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
-
Nicotiana tabacum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
I468A
the mutant produces significant amounts 1beta-hydroxy-5-epiaristolochene, but negligible amounts of capsidiol from 5-epiaristolochene
Nicotiana tabacum
S368A
the mutant retains its ability to fully convert 5-epiaristolochene to capsidiol, but the turnover rates for capsidiol formation is 3-13times lower compared with that of the wild type enzyme and is also able to convert 1beta-hydroxy-5-epiaristolochene to capsidiol with kcat values comparable with that of wild type enzyme, but with Km values for 1beta-hydroxy-5-epiaristolochene 4-6times greater compared with that of wild type
Nicotiana tabacum
S368C
the mutant exhibits wild type catalytic efficiency for 1beta-hydroxy-5-epiaristolochene biosynthesis, but is devoid of the successive hydroxylation activity for capsidiol biosynthesis
Nicotiana tabacum
S368F
devoid of any hydroxylase activity for 5-epiaristolochene or 1beta-hydroxy-5-epiaristolochene
Nicotiana tabacum
S368I
devoid of any hydroxylase activity for 5-epiaristolochene or 1beta-hydroxy-5-epiaristolochene
Nicotiana tabacum
S368T
the mutant retains its ability to fully convert 5-epiaristolochene to capsidiol, but the turnover rates for capsidiol formation is 3-13times lower compared with that of the wild type enzyme and is also able to convert 1beta-hydroxy-5-epiaristolochene to capsidiol with kcat values comparable with that of wild type enzyme, but with Km values for 1beta-hydroxy-5-epiaristolochene 4-6times greater compared with that of wild type
Nicotiana tabacum
S368V
the mutant exhibits wild type catalytic efficiency for 1beta-hydroxy-5-epiaristolochene biosynthesis, but is devoid of the successive hydroxylation activity for capsidiol biosynthesis, the mutant catalyzes the relative equal biosynthesis of 1beta-hydroxy-5-epiaristolochene, 2beta-hydroxy-5-epiaristolochene, and 3beta-hydroxy-5-epiaristolochene from 5-epiaristolochene with wild type efficiency and converts about 1.5% of these monohydroxylated products to their respective ketone forms
Nicotiana tabacum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
dimethyl sulfoxide
conversion of 5-epiaristolochene to capsidiol activity is inhibited at concentrations above 10% (v/v) dimethyl sulfoxide
Nicotiana tabacum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00174
-
1beta-hydroxy-5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.0062
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.00997
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.01518
-
5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.01672
-
5-epiaristolochene
mutant enzyme S386V, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.01918
-
5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.02124
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme I486A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.02267
-
5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.03863
-
5-epiaristolochene
mutant enzyme S386C, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.0688
-
5-epiaristolochene
mutant enzyme I486A, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1beta-hydroxy-5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
the catalytic efficiency for 1beta-hydroxy-5-epiaristolochene is about 10times greater than that for 3alpha-hydroxy-5-epiaristolochene
712358
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
3alpha-hydroxy-5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
the catalytic efficiency for 3alpha-hydroxy-5-epiaristolochene is about 10times lower than that for 1beta-hydroxy-5-epiaristolochene
712358
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
-
712358
Nicotiana tabacum
capsidiol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + [reduced NADPH-hemoprotein reductase] + O2
the release of an 1beta-hydroxy-5-epiaristolochene intermediate at high 5-epiaristolochene concentrations and a 10fold catalytic preference for 1beta-hydroxy-5-epiaristolochene versus 3alpha-hydroxy-5-epiaristolochene is indicative of a preferred reaction order of hydroxylation at C-1, followed by that at C-3
712358
Nicotiana tabacum
1beta-hydroxy-5-epiaristolochene + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.037
-
5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.132
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme I486A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.154
-
5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.278
-
5-epiaristolochene
mutant enzyme S386C, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.293
-
5-epiaristolochene
mutant enzyme I486A, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.482
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.493
-
5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.553
-
5-epiaristolochene
mutant enzyme S386V, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.581
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
0.582
-
1beta-hydroxy-5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.4
-
5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
4.3
-
5-epiaristolochene
mutant enzyme I486A, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
6.2
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme I486A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
6.8
-
5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
7.2
-
5-epiaristolochene
mutant enzyme S386C, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
25.7
-
5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
33.1
-
5-epiaristolochene
mutant enzyme S386V, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
48.3
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
93.7
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
334.4
-
1beta-hydroxy-5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
2.4
-
5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
4.3
-
5-epiaristolochene
mutant enzyme I486A, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
6.2
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme I486A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
6.8
-
5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
7.2
-
5-epiaristolochene
mutant enzyme S386C, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
25.7
-
5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
33.1
-
5-epiaristolochene
mutant enzyme S386V, the reaction product is 1beta-hydroxy-5-epiaristolochene, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
48.3
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368T, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
93.7
-
1beta-hydroxy-5-epiaristolochene
mutant enzyme S368A, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
334.4
-
1beta-hydroxy-5-epiaristolochene
wild type enzyme, the reaction product is capsidiol, in 100 mM Tris-HCl (pH 7.5), at 30°C
Nicotiana tabacum
Other publictions for EC 1.14.14.149
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745907
Lee
Expansion of sesquiterpene bi ...
Capsicum annuum
New Phytol.
215
1132-1143
2017
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1
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4
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1
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712358
Takahashi
Kinetic and molecular analysis ...
Nicotiana tabacum
J. Biol. Chem.
280
3686-3696
2005
1
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1
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7
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10
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1
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4
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10
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1
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1
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1
1
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7
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1
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10
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4
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10
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10
10
710995
Greenhagen
Probing sesquiterpene hydroxyl ...
Nicotiana tabacum
Arch. Biochem. Biophys.
409
385-394
2003
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1
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6
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2
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8
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1
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1
1
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6
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8
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710993
Ralston
Cloning, heterologous expressi ...
Nicotiana tabacum
Arch. Biochem. Biophys.
393
222-235
2001
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2
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4
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1
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1
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2
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1
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2
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1
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1
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