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Literature summary for 1.14.14.15 extracted from

  • Lin, S.; van Lanen, S.G., Shen, B.
    Regiospecific chlorination of (S)-beta-tyrosyl-S-carrier protein catalyzed by SgcC3 in the biosynthesis of the enediyne antitumor antibiotic C-1027 (2007), J. Am. Chem. Soc., 129, 12432-12438.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.14.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces globisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl-[peptidyl-carrier protein SgcC2] + FADH2 + O2 Streptomyces globisporus
-
 (3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
 ?
additional information Streptomyces globisporus SgcC3 also efficiently catalyzes bromination but not fluorination or iodination ?
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces globisporus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography Streptomyces globisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3S)-3-amino-3-(3-chloro-4-hydroxyphenyl)propanoyl-[peptidyl-carrier protein SgcC2] + FADH2 + O2
-
 Streptomyces globisporus (3S)-3-amino-3-(3-chloro-4,5-dihydroxyphenyl)propanoyl-[peptidyl-carrier protein SgcC2] + FAD + H2O
-
 ?
(R)-alpha-tyrosyl-[SgcC2] + FADH2 + O2 SgcC3 can utilize both (S)- and (R)-alpha-tyrosyl-[SgcC2] but not 3-hydroxy-alpha-tyrosyl-[SgcC2] as a substrate Streptomyces globisporus ?
-
 ?
(S)-alpha-tyrosyl-[SgcC2] + FADH2 + O2 SgcC3 can utilize both (S)- and (R)-alpha-tyrosyl-[SgcC2] but not 3-hydroxy-alpha-tyrosyl-[SgcC2] as a substrate Streptomyces globisporus ?
-
 ?
additional information SgcC3 also efficiently catalyzes bromination but not fluorination or iodination Streptomyces globisporus ?
-
 ?
additional information no activity with 3-hydroxy-alpha-tyrosyl-S-SgcC2 Streptomyces globisporus ?
-
 ?

Synonyms

Synonyms Comment Organism
SgcC3
-
 Streptomyces globisporus

Cofactor

Cofactor Comment Organism Structure
FAD dependent on Streptomyces globisporus