BRENDA - Enzyme Database show
show all sequences of 1.14.14.150

Biosynthesis of costunolide, dihydrocostunolide, and leucodin. Demostration of cytochrome P450-catalyzed formation of the lactone ring present in sesquiterpene lactones of chicory

de Kraker, J.W.; Franssen, M.C.R.; Joerink, M.; de Groot, A.; Bouwmeester, H.J.; Plant Physiol. 129, 257-268 (2002)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
CO
the inhibition in the presence of 90% (v/v) CO and 10% (v/v) O2 is reversed to some extent by irradiation with blue light (450 nm)
Cichorium intybus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Cichorium intybus
(+)-costunolide synthase is a cytochrome P450 enzyme
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cichorium intybus
-
cultivar Focus
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Cichorium intybus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
657039
Cichorium intybus
(+)-costunolide + [oxidized NADPH-hemoprotein reductase] + H2O
(+)-costunolide is detected as its Cope rearrangement product dehydrosaussurea lactone
-
-
ir
additional information
(+)-costunolide synthase is a cytochrome P450 enzyme
657039
Cichorium intybus
?
-
-
-
-
additional information
(+)-costunolide synthase is not capable of converting alpha-costic acid and elema-1,3,11(13)-trien-12-oic acid
657039
Cichorium intybus
?
-
-
-
-
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
CO
the inhibition in the presence of 90% (v/v) CO and 10% (v/v) O2 is reversed to some extent by irradiation with blue light (450 nm)
Cichorium intybus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Cichorium intybus
(+)-costunolide synthase is a cytochrome P450 enzyme
?
-
-
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Cichorium intybus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
657039
Cichorium intybus
(+)-costunolide + [oxidized NADPH-hemoprotein reductase] + H2O
(+)-costunolide is detected as its Cope rearrangement product dehydrosaussurea lactone
-
-
ir
additional information
(+)-costunolide synthase is a cytochrome P450 enzyme
657039
Cichorium intybus
?
-
-
-
-
additional information
(+)-costunolide synthase is not capable of converting alpha-costic acid and elema-1,3,11(13)-trien-12-oic acid
657039
Cichorium intybus
?
-
-
-
-
Other publictions for EC 1.14.14.150
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745633
Farzadfar
Magnesium and manganese inter ...
Tanacetum parthenium
J. Plant Physiol.
202
10-20
2016
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746141
Eljounaidi
Cytochrome P450s from Cynara ...
Cynara cardunculus var. altilis
Plant Sci.
223
59-68
2014
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727877
Ikezawa
Lettuce costunolide synthase ( ...
Helianthus annuus, Lactuca sativa
J. Biol. Chem.
286
21601-21611
2011
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2
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728554
Liu
Reconstitution of the costunol ...
Cichorium intybus
PLoS ONE
6
e23255
2011
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5
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657039
de Kraker
Biosynthesis of costunolide, d ...
Cichorium intybus
Plant Physiol.
129
257-268
2002
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