BRENDA - Enzyme Database show
show all sequences of 1.14.14.150

Reconstitution of the costunolide biosynthetic pathway in yeast and Nicotiana benthamiana

Liu, Q.; Majdi, M.; Cankar, K.; Goedbloed, M.; Charnikhova, T.; Verstappen, F.W.; de Vos, R.C.; Beekwilder, J.; van der Krol, S.; Bouwmeester, H.J.; PLoS ONE 6, e23255 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in yeast cells and in Nicotiana benthamiana leaves
Cichorium intybus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
Cichorium intybus
-
(+)-costunolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cichorium intybus
G3GBK0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
root
-
Cichorium intybus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
728554
Cichorium intybus
(+)-costunolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Cichorium intybus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in yeast cells and in Nicotiana benthamiana leaves
Cichorium intybus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome P450
-
Cichorium intybus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
Cichorium intybus
-
(+)-costunolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
root
-
Cichorium intybus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2
-
728554
Cichorium intybus
(+)-costunolide + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Other publictions for EC 1.14.14.150
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745633
Farzadfar
Magnesium and manganese inter ...
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202
10-20
2016
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-
1
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2
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1
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3
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1
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1
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1
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1
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1
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1
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1
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1
1
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746141
Eljounaidi
Cytochrome P450s from Cynara ...
Cynara cardunculus var. altilis
Plant Sci.
223
59-68
2014
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1
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6
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727877
Ikezawa
Lettuce costunolide synthase ( ...
Helianthus annuus, Lactuca sativa
J. Biol. Chem.
286
21601-21611
2011
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2
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2
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5
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2
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2
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2
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728554
Liu
Reconstitution of the costunol ...
Cichorium intybus
PLoS ONE
6
e23255
2011
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1
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-
-
-
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1
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5
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1
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1
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1
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1
1
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1
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1
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1
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657039
de Kraker
Biosynthesis of costunolide, d ...
Cichorium intybus
Plant Physiol.
129
257-268
2002
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1
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1
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2
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1
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3
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1
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1
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