BRENDA - Enzyme Database show
show all sequences of 1.14.14.151

Functional characterization of premnaspirodiene oxygenase, a cytochrome P450 catalyzing regio- and stereo-specific hydroxylations of diverse sesquiterpene substrates

Takahashi, S.; Yeo, Y.S.; Zhao, Y.; O'Maille, P.E.; Greenhagen, B.T.; Noel, J.P.; Coates, R.M.; Chappell, J.; J. Biol. Chem. 282, 31744-31754 (2007)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in WAT11 yeast strain
Hyoscyamus muticus
Engineering
Amino acid exchange
Commentary
Organism
A484I
the mutation results in a catalytically compromised enzyme
Hyoscyamus muticus
V366S
the mutation dramatically reduces overall enzyme activity
Hyoscyamus muticus
V480I/A484I
mutant with improved catalytic efficiency
Hyoscyamus muticus
V480S
the mutation improves the kcat for the conversion of (-)-vetispiradiene to solavetivol about 2fold
Hyoscyamus muticus
V482I
mutant with improved catalytic efficiency; the mutation improves the kcat for the conversion of (-)-vetispiradiene to solavetivol about 2fold
Hyoscyamus muticus
V482I/A484I
the mutant possesses a 5fold improvement in its catalytic efficiency for nootkatol biosynthesis and a 10fold improvement for 2beta-hydroxy-epiaristolochene biosynthesis
Hyoscyamus muticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0025
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0033
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0043
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0062
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0069
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0074
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0084
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0121
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.013
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.014
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0179
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0192
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Hyoscyamus muticus
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Hyoscyamus muticus
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Hyoscyamus muticus
A6YIH8
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-vetispiradiene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
solavetivol + [oxidized NADPH-hemoprotein reductase] + H2O
solavetivol is also named premnaspirodien-2alpha-ol or (2S,4R,5S,7R)-spirovetiva-1(10),11(12)-dien-2-ol
-
-
?
4-epi-eremophilene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
4-epieremophilen-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
substrate affinity for 5-epiaristolochene is 2-4times lower than that for valencene or (-)-premnaspirodiene, respectively. HPO catalyzes the conversion of 5-epiaristolochene to four mono-hydroxylated products with 2beta-hydroxy-epiaristolochene comprising greater than 80% of the reaction products, 1beta-hydroxy-epiaristolochene about 5%, 3alpha-hydroxy-epiaristolochene less than 2%, and an unknown mono-hydroxylated product of about 20%
712360
Hyoscyamus muticus
2beta-hydroxy-5-epiaristolochene + 1beta-hydroxy-5-epiaristolochene + 3alpha-hydroxy-5-epiaristolochene + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
cedr-8-ene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
cedr-8-en-15-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene
712360
Hyoscyamus muticus
?
-
-
-
-
additional information
the enzyme fails to convert nootkatol to nootkatone
712360
Hyoscyamus muticus
?
-
-
-
-
valencene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
712360
Hyoscyamus muticus
alpha-nootkatol + beta-nootkatol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.7
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1.4
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1.9
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
2.1
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
2.8
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
3.8
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
5.1
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
5.7
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
12.7
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
15.9
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
20.7
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in WAT11 yeast strain
Hyoscyamus muticus
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
A484I
the mutation results in a catalytically compromised enzyme
Hyoscyamus muticus
V366S
the mutation dramatically reduces overall enzyme activity
Hyoscyamus muticus
V480I/A484I
mutant with improved catalytic efficiency
Hyoscyamus muticus
V480S
the mutation improves the kcat for the conversion of (-)-vetispiradiene to solavetivol about 2fold
Hyoscyamus muticus
V482I
mutant with improved catalytic efficiency; the mutation improves the kcat for the conversion of (-)-vetispiradiene to solavetivol about 2fold
Hyoscyamus muticus
V482I/A484I
the mutant possesses a 5fold improvement in its catalytic efficiency for nootkatol biosynthesis and a 10fold improvement for 2beta-hydroxy-epiaristolochene biosynthesis
Hyoscyamus muticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0025
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0033
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0043
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0062
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0069
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0074
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0084
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0121
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.013
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.014
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0179
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.0192
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
microsome
-
Hyoscyamus muticus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Hyoscyamus muticus
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(-)-vetispiradiene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
solavetivol + [oxidized NADPH-hemoprotein reductase] + H2O
solavetivol is also named premnaspirodien-2alpha-ol or (2S,4R,5S,7R)-spirovetiva-1(10),11(12)-dien-2-ol
-
-
?
4-epi-eremophilene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
4-epieremophilen-2beta-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
5-epiaristolochene + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
substrate affinity for 5-epiaristolochene is 2-4times lower than that for valencene or (-)-premnaspirodiene, respectively. HPO catalyzes the conversion of 5-epiaristolochene to four mono-hydroxylated products with 2beta-hydroxy-epiaristolochene comprising greater than 80% of the reaction products, 1beta-hydroxy-epiaristolochene about 5%, 3alpha-hydroxy-epiaristolochene less than 2%, and an unknown mono-hydroxylated product of about 20%
712360
Hyoscyamus muticus
2beta-hydroxy-5-epiaristolochene + 1beta-hydroxy-5-epiaristolochene + 3alpha-hydroxy-5-epiaristolochene + 3 [oxidized NADPH-hemoprotein reductase] + 3 H2O
-
-
-
?
cedr-8-ene + [reduced NADPH-hemoprotein reductase] + O2
-
712360
Hyoscyamus muticus
cedr-8-en-15-ol + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
additional information
HPO also catalyzes the equivalent regio-specific (C-2) hydroxylation of several eremophilane-type (decalin ring system) sesquiterpenes, such as with 5-epi-aristolochene
712360
Hyoscyamus muticus
?
-
-
-
-
additional information
the enzyme fails to convert nootkatol to nootkatone
712360
Hyoscyamus muticus
?
-
-
-
-
valencene + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
712360
Hyoscyamus muticus
alpha-nootkatol + beta-nootkatol + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
0.7
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1.4
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1.9
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
2.1
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
2.8
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
3.8
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
5.1
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
5.7
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
12.7
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
15.9
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
20.7
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
60
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
80
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
150
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
260
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
280
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
300
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
610
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
650
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
740
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1310
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1510
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1590
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
60
-
5-epiaristolochene
wild type enzyme, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
80
-
5-epiaristolochene
mutant enzyme V482I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
150
-
(-)-vetispiradiene
wild type enzyme, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
260
-
valencene
wild type enzyme, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
280
-
5-epiaristolochene
mutant enzyme V480I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
300
-
valencene
mutant enzyme V480I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
610
-
(-)-vetispiradiene
mutant enzyme V482I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
650
-
5-epiaristolochene
mutant enzyme V482I/A484I, product is 2beta-hydroxy-epiaristolochene, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
740
-
valencene
mutant enzyme V482I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1310
-
valencene
mutant enzyme V482I/A484I, product is beta-nootkatol, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1510
-
(-)-vetispiradiene
mutant enzyme V480I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
1590
-
(-)-vetispiradiene
mutant enzyme V482I/A484I, in 100 mM Tris-HCl, pH 7.5, at 30C
Hyoscyamus muticus
Other publictions for EC 1.14.14.151
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745627
Nino
-
Overexpression of rice premna ...
Oryza sativa Japonica Group
J. Plant Biotechnol.
43
422-431
2016
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1
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1
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1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
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-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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1
1
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745687
Wriessnegger
Production of the sesquiterpe ...
Hyoscyamus muticus
Metab. Eng.
24
18-29
2014
-
-
1
-
1
-
-
-
-
-
-
2
-
10
-
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-
-
-
-
-
-
2
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-
-
-
-
-
-
1
-
-
-
-
-
1
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712360
Takahashi
Functional characterization of ...
Hyoscyamus muticus
J. Biol. Chem.
282
31744-31754
2007
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