BRENDA - Enzyme Database show
show all sequences of 1.14.14.152

Licorice beta-amyrin 11-oxidase, a cytochrome P450 with a key role in the biosynthesis of the triterpene sweetener glycyrrhizin

Seki, H.; Ohyama, K.; Sawai, S.; Mizutani, M.; Ohnishi, T.; Sudo, H.; Akashi, T.; Aoki, T.; Saito, K.; Muranaka, T.; Proc. Natl. Acad. Sci. USA 105, 14204-14209 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Spodoptera frugiperda 9 (Sf9) insect cells
Glycyrrhiza uralensis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Glycyrrhiza uralensis
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
not detected in leaves and stems
Glycyrrhiza uralensis
-
root
-
Glycyrrhiza uralensis
-
stolon
-
Glycyrrhiza uralensis
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
30-hydroxy-beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
716744
Glycyrrhiza uralensis
11-deoxoglycyrrhetinic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
two-step oxidation at C-11 with 11alpha-hydroxy-beta-amyrin as intermediate product
716744
Glycyrrhiza uralensis
11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Spodoptera frugiperda 9 (Sf9) insect cells
Glycyrrhiza uralensis
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
not detected in leaves and stems
Glycyrrhiza uralensis
-
root
-
Glycyrrhiza uralensis
-
stolon
-
Glycyrrhiza uralensis
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
30-hydroxy-beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
-
716744
Glycyrrhiza uralensis
11-deoxoglycyrrhetinic acid + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
beta-amyrin + [reduced NADPH-hemoprotein reductase] + O2
two-step oxidation at C-11 with 11alpha-hydroxy-beta-amyrin as intermediate product
716744
Glycyrrhiza uralensis
11-oxo-beta-amyrin + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
General Information
General Information
Commentary
Organism
metabolism
CYP88D6 is a beta-amyrin 11-oxidase in the glycyrrhizin pathway
Glycyrrhiza uralensis
General Information (protein specific)
General Information
Commentary
Organism
metabolism
CYP88D6 is a beta-amyrin 11-oxidase in the glycyrrhizin pathway
Glycyrrhiza uralensis
Other publictions for EC 1.14.14.152
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
716744
Seki
Licorice beta-amyrin 11-oxidas ...
Glycyrrhiza uralensis
Proc. Natl. Acad. Sci. USA
105
14204-14209
2008
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