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Literature summary for 1.14.14.154 extracted from

  • O'Brien, M.; Chantha, S.C.; Rahier, A.; Matton, D.P.
    Lipid signaling in plants. Cloning and expression analysis of the obtusifoliol 14alpha-demethylase from Solanum chacoense Bitt., a pollination- and fertilization-induced gene with both obtusifoliol and lanosterol demethylase activity (2005), Plant Physiol., 139, 734-749.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
gene CYP51, DNA and amino acid sequence determination and analysis, sequence comparison, expression in Saccharomyces cerevisiae, functional complementation of the DELTAerg11 enzyme deficient yeast mutant strain to 81% cell viability Solanum chacoense

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Solanum chacoense 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55200
-
 x * 55200, amino acid sequence caculation Solanum chacoense

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2 Solanum chacoense
-
 4,4-dimethylzymosterol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 Solanum chacoense the enzyme is involved in biosynthesis of sitosterol and brassinosteroids, pathways overview 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum chacoense Q673E9 gene CYP51
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf mature, weak enzyme expression Solanum chacoense
-
additional information tissue- and development-specific expression pattern, in situ hybridization, no enzyme expression in stems, petals, stamens, pollen, and in-vitro-grown pollen tubes, overview, enzyme expression is regulated both by pollination and by fertilization Solanum chacoense
-
pistil ovules and ovaries without pericarp, transient increase in enzyme expression around 72 h postpollination pistils, weak enzyme expression in unpollinated pistils on the day of anthesis Solanum chacoense
-
root weak enzyme expression Solanum chacoense
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
lanosterol + [reduced NADPH-hemoprotein reductase] + O2
-
 Solanum chacoense 4,4-dimethylzymosterol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2
-
 Solanum chacoense 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
obtusifoliol + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in biosynthesis of sitosterol and brassinosteroids, pathways overview Solanum chacoense 4alpha-methyl-5alpha-ergost-8,14,24(28)-trien-3beta-ol + formate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 55200, amino acid sequence caculation Solanum chacoense

Synonyms

Synonyms Comment Organism
CYP51G1
-
 Solanum chacoense
lanosterol demethylase
-
 Solanum chacoense
obtusifoliol 14alpha-demethylase
-
 Solanum chacoense
sterol 14alpha-demethylase
-
 Solanum chacoense

pI Value

Organism Comment pI Value Maximum pI Value
Solanum chacoense amino acid sequence caculation
-
 8.7